急性右心功能衰竭(acute right heart failure,ARHF),又称急性右心室综合征(acute right heartsvndrome,ARHS)是由于右心扩张和(或)功能障碍导致体循环充血、组织低灌注、低血压、心肾综合征或心肝综合征等急性进展性综合征[1-3]。右心衰竭尚没有明确的定量标准,目前倾向性的标准为右心室心脏指数(cardiac index,CI)<2.5L/(min·m2)和右心室充盈压>8mmHg(1mmHg-O.133kPa)。右心衰竭的诊断至少具备2个特征:与右心衰竭一致的症状与体征,或右心心脏结构和(或)功能异常,或右心压力增加的客观依据。
Background：The incidence of cancer,diabetes,and autoimmune diseases has been increasing.Furthermore,there are more and more patients with solid organ transplants.The survival rate of these immunocompromised individuals is extremely low when they are severely hit-on.In this study,we established cardiac arrest cardiopulmonary resuscitation （CPR） model in severe combined immunodeficient （SCID） mice,analyzed the expression and activation of mitochondrial autophagy and NLRP3 inflammasome/caspase-1,and explored mitochondrial repair and inflammatory injury in immunodeficiency individual during systemic ischemia-reperfusion injury.Methods：A potassium chloride-induced cardiac arrest model was established in C57BL/6 and nonobese diabetic/SCID （NOD/SCID） mice.One hundred male C57BL/6 mice and 100 male NOD/SCID mice were randomly divided into five groups （control,2 h post-CPR,12 h post-CPR,24 h post-CPR,and 48 h post-CPR）.A temporal dynamic view of alveolar epithelial cells,macrophages,and neutrophils from bronchoalveolar lavage fluid （BALF） was obtained using Giemsa staining.Spatial characterization ofphenotypic analysis of macrophages in the lung interstitial tissue was analyzed by flow cytometry.The morphological changes ofmitochondria 48 h after CPR were studied by transmission electron microscopy and quantified according to the Flameng grading system.Western blotting analysis was used to detect the expression and activation of the markers of mitochondrial autophagy,NLRP3 inflammasome,and caspase-1.Results：（1） In NOD/SCID mice,macrophages were disintegrated in BALF,and many alveolar epithelial cells were shed at 48 h after resuscitation.Compared with C57BL/6 mice,the ratio of macrophages/total cells peaked at 12 h and was significantly higher in NOD/SCID mice （31.17 ± 4.13 vs.49.69 ± 2.43,t =14.46,P =0.001）.After 24 h,the results showed a downward trend.Furthermore,a large number of macrophages were disintegrated in the BALF.（2） Mitochondrial autophagy was present in both C57BL/6 a
Jing-Jun LyuJawahar L MehtaYi LiLu YeSheng-Nan SunHong-Shuang SunJia-Chang LiDong-Mei ZhangJie Wei
