A method for determination of fatty acids(FA) compositions in human serum by gas chromatography time-of flight mass spectrometry(GC-TOF MS) method was established.The FA in serum were directly transformed into the fatty acid methyl esters by 2.5% H2SO4-methanol.Heptadecanoic acid methyl esters were used as internal standard.The detection and separation of all the FA were performed within 33 min.The established method has the characteristic of simple operation,rapid analysis,high accuracy,large amount of information and safety.So it is suitable for the scientific research to the disease correlating FA.
Liquid chromatography-mass spectrometry(LC-MS) was combined by high resolution of high-liquid chromatography and high sensitivity,high specificity of mass spectrometry,which not only can separate the complicated mixtures,but also doesn’t need more complicated pre-treatment for samples.The qualitative and quantitative analysis of quercetin was implemented by the information of the molecular structure and fragment.In this paper,the LC-MS/MS method was established for determination of quercetin in plasma.
