[Objective] The aim was to investigate the effects of sound wave on propagation of Chlorella pyrenoidosa to explore the optimal frequency for Chlorella pyrenoidosa.[Method] In the research,Chlorella pyrenoidosa was cultured for 7 d with sound waves at different frequencies and a control group was set to study effects of sound wave at different frequencies on growth of Chlorella pyrenoidosa.[Result] Growth of Chlorella pyrenoidosa was significantly improved by sound wave,especially for wave at 400 Hz.[Conclusion] Chlorella pyrenoidosa propagation would be promoted by sound wave at certain frequencies.