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林慧敏

作品数:2 被引量:11H指数:2
供职机构:天津中医药大学更多>>
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毛蕊花糖苷通过激活PI3K/AKT通路促进成年小鼠神经干细胞增殖被引量:9
2016年
目的观察毛蕊花糖苷对原代成年小鼠神经干细胞增殖的影响,并探讨其可能的作用机制。方法从成年C57BL/6小鼠脑室下区分离、培养原代神经干细胞,并通过神经干细胞标志蛋白Nestin免疫荧光染色,对分离得到的神经干细胞进行鉴定。不同浓度毛蕊花糖苷(5、10、20、40μmol·L^(-1))在无有丝分裂原(EGF/bF GF)的条件下处理细胞24 h。采用CCK-8法检测细胞活力,免疫组化法计数BrdU阳性细胞率,检测细胞增殖能力,Western blot方法检测给药后神经干细胞Akt的磷酸化水平。结果毛蕊花糖苷在无有丝分裂原存在的条件下,能明显促进神经干细胞的增殖,并明显提高p-Akt的表达。而在加入PI3K/AKT信号通路阻断剂LY294002后,这一作用被明显抑制。结论毛蕊花糖苷对体外培养的神经干细胞具有明显促增殖作用,该作用机制可能与化合物激活Akt通路有关。
林慧敏段伟兵邵瑞韩立峰朱彦高秀梅王彧
关键词:毛蕊花糖苷成年小鼠神经干细胞脑室下区增殖AKT通路
A summary of acupuncture standardization in Australia, Korea, Japan and the USA被引量:2
2017年
Objective By comparing the focus of acupuncture standards, management modes of acupuncture in certain areas are summarized to provide a reference for institutions and regions when formulating suitable standards for local acupuncture services. Methods Japan, South Korea, the USA and Australia were selected as the representative countries. The standards language were limited to English, Japanese and South Korean. The websites of the national standards bodies and the official websites for the acupuncture association/ institute of these four countries were searched systematically. The laws of acupuncture were included in the study. Results Technical standards made up the most of the standard system for acupuncture in Japan, basic standards accounted for the most of the system in South Korea, while management standards accounted for the largest proportion of acupuncture standard systems in the USA and Australia. Conclusion Japan and South Korea, which use acupuncture mainly due to cultural and historical influences, place emphasis on establishing technical and basic standards. The USA and Australia, which use acupuncture as a complementary therapy, place emphasis on establishing management standards of acupuncture.
杨馥铭杨毅渡边大佑金光郭义赵雪郭永明陈泽林林慧敏高旸
关键词:ACUPUNCTURELEGISLATIONAUSTRALIAJAPAN
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