您的位置: 专家智库 > >

文献类型

  • 3篇中文期刊文章

领域

  • 3篇农业科学

主题

  • 1篇抑菌
  • 1篇抑菌率
  • 1篇抑菌浓度
  • 1篇植物源
  • 1篇植物源农药
  • 1篇嗜热链球菌
  • 1篇农药
  • 1篇最低抑菌浓度
  • 1篇物源农药
  • 1篇胞外多糖
  • 1篇RATE
  • 1篇EIGHT
  • 1篇FUNGUS
  • 1篇IR
  • 1篇MIC
  • 1篇纯化
  • 1篇ANTIFU...

机构

  • 3篇山西大学
  • 2篇太原市环境保...

作者

  • 3篇王兴华
  • 3篇高磊
  • 2篇李颖
  • 1篇陈真真
  • 1篇张宁波
  • 1篇陈悦

传媒

  • 2篇安徽农业科学
  • 1篇Plant ...

年份

  • 3篇2010
3 条 记 录,以下是 1-3
排序方式:
8种植物源农药的提取工艺及其抑制真菌作用研究被引量:3
2010年
[目的]研究黄连、丁香等8种药用植物抑菌成分的最佳提取工艺及对2种植物病原菌的抑制作用。[方法]采用水提、乙醇提、丙酮提3种提取方法提取各种药用植物有效成分,采用药物平板稀释法测定药用植物提取物的最低抑菌浓度(MIC)和抑菌率。[结果]抑制2种植物病原菌的最优药用植物及提取方法分别为:抑制地黄枯萎病菌采用丁香乙醇提和丙酮提,肉桂乙醇提,此时MIC达到4 mg/ml,抑制肉苁蓉致病菌采用丁香乙醇提和丙酮提,肉桂丙酮提,此时MIC达到4 mg/ml。[结论]8种药用植物的乙醇和丙酮提取物对供试菌种的抑制作用明显优于水提物。
高磊谢宇琪王兴华李颖
关键词:植物源农药最低抑菌浓度抑菌率
嗜热链球菌胞外多糖纯化条件的研究
2010年
[目的]探索一种分离纯化嗜热链球菌胞外多糖的途径。[方法]采用嗜热链球菌为材料,以MRS培养基为基础培养基,生产嗜热链球菌胞外多糖。采用酶法、TCA法、Sevag法、酶+TCA法、酶+Sevag法、TCA+Sevag法、酶+TCA+Sevag法研究对嗜热链球菌胞外多糖去蛋白的影响,对得到的粗多糖用DEAE-Cellulose离子交换柱和葡聚糖G-100凝胶柱进行了分离,并用紫外光谱扫描对其的纯度进行鉴定。[结果]粗多糖用蒸馏水和0.1 mol/L NaC l通过DEAE-Cellulose离子交换柱可以分为中性多糖和酸性多糖,用葡聚糖G-100凝胶柱对酸性多糖的纯度进行了鉴定,得到单一的峰,紫外光谱扫描对酸性多糖进行全波长扫描,酸性多糖中不含蛋白质和核酸,证明得到的酸性多糖为纯多糖。[结论]酶+Sevag法为最佳去蛋白法。
王兴华张宁波陈真真高磊陈悦
关键词:嗜热链球菌胞外多糖纯化
Extraction Technology of Eight Botanical Pesticides and Their Antifungal Activities on Fungus
2010年
[ Objective] The optimum extraction technology of antifungal ingredients from eight medicinal plants such as coptis root and clove, etc. and their antifungal effects on two species of plant pathogens were studied. [ Method] The effective ingredients of medicinal plants were extracted by water, ethanol and acetone. The minimum inhibitory concentration (MIC) and antifungal rate of extracts from medicinal plants were determined using plate diluting method. [Resets] The optimum medicinal plants and the extraction methods inhibited two plant pathogens were as follows: clove extracted by ethanol and acetone, and cinnamon extracted by ethanol could be adopted to inhibit Fusarium oxysporum Schl., MIC at that mo- ment reached 4 mg/ml. Clove extracted by ethanol and acetone, and cinnamon extracted by ethanol could be adopted to inhibit the pathogen of Cistanche deserticola, MIC at that moment reached 4 mg/ml. [ Conclusion] The antifungal effects of ethanol and acetone extracts on tested patho- gens were obviously superior than that of aqueous extracts.
高磊谢宇琪王兴华李颖
关键词:MIC
共1页<1>
聚类工具0