To determine the optimal time for harvesting the seeds of Gui 39722, which is the female parent of a newly-developed maize hybrid Guidan 0810, the cobs of Gui 39722 were harvested 31 （the first stage）, 34 （the second stage）, 37 （the third stage）, 40 （the fourth stage）, 43 （the fifth stage） and 46 （the sixth stage） d after pollination and oven dried at 38 ~C to constant weight. Then, the 1 000-ker- nel weight, germination rate and the agronomic traits of three-leaf seedlings were measured and compared. The results showed that the seeds of Gui 39722 harvest- ed 43 d after pollination （the fifth stage） had the greatest germination rate, seedling height, vigor index, root dry weight, shoot dry weight and germination potential, and high 1 000-kernel weight （very close to the highest level）, root length, number of roots per seedling and root-shoot ratio. Therefore, 43 d after pollination was the op- timal time for harvesting the seeds of Gui 39722, when the effective accumulated temperature after pollination reached 780.7 ℃ and the ear leaf dried. The seeds harvested at this stage had the highest yield and the best quality. Field sampling revealed that 89.00% to 95.00% （with an average of 91.15%） of the seedlings of six stages of seeds grew well.
