The genetic diversity among 32 accessions of Hordeum bogdanii Wilensky native to Xinjiang, China, was evaluated by 22 STS_PCR primer sets derived from RFLP clones of the wheat ( Triticum aestivum L.) or barley ( Hordeum vulgare L.) mapping. Out of the 22 STS_PCR markers, only three markers gave products which did not generate polymorphic bands upon digestion with Hin fⅠ, Hha Ⅰ, Hae Ⅲ and Rsa Ⅰ, while 19 out of 22 markers (86.4%) and 46 out of 88 marker/enzyme combinations (52.3%) revealed polymorphisms. Among the 32 H. bogdanii accessions, a total of 315 bands were observed in 88 STS_PCR marker/enzyme combinations, with 3.6 bands each. One hundred and twenty_three out of 315 bands (39.0%) were polymorphic, among which 1 to 6 polymorphic bands were generated by each polymorphic marker/enzyme combination. The STS_PCR_based genetic diversity index ( GD ) among 32 H. bogdanii accessions ranged between 0.078 to 0.352, with a mean of 0.198. Based on the GD matrix, a dendrogram showing the genetic relationships between accessions was constructed using the unweighted pair_group method with arithmetic average (UPGMA). Results showed that all 32 accessions could be distinguished by STS_PCR markers. The accessions originated from the same region were distributed within different groups or subgroups. This study indicates that the genetic diversity of H. bogdanii is not closely correlated with the geographical distribution.
