Ribavirin production by the mixed fermentation of Bacillus substilis TM903 and Corynebacterium glutamicum TL1105 was studied.The inoculum size of TL1105 and the optimal addition of KH2PO4, MnSO4, 1H-1,2,4-triazole-3-carboxamide (TCA) in the fermentation medium were established initially.Then, the factorial design by MINITAB software was employed to optimize the adding mode of glucose, KH2PO4, MnSO4, TCA, which suggested that MINITAB software was the more effective tool in the culture medium optimization.Under the optimal conditions, the yield of ribavirin was 4.69 g·L-1 after 60 h fermentation with glucose added one-off and KH2PO4, Mn2+and TCA fed in batches.
