[Objective] The aim of this study was to investigate the effect of space flight factors on plant biomass in the generation of alfalfa carried by the satellite.[Method]Seeds from three lines of alfalfa were carried by the seed-breeding satellite Shijian-8.After the satellite returned to the ground,stem diameter,primary branch number and current-year individual biomass of alfalfa were studied.[Result]After space flight,primary branch number and current-year individual biomass of alfalfa increased significantly,while the stem diameter had no significant change.Using the value over(the mean value of control + three standard deviation)as a criterion to screen,the variants with enlarged stem diameter,increased primary branch number and individual biomass was two,five and twelve respectively.[Conclusion]The obtained variants can be used in the variety improvement of alfalfa and its new variety breeding,but whether its favorable variation can inherit stably to the progenies needs further study.