[Objective] The aim of this study was to investigate the content changes and their correlations of the photosynthetic pigment,phenols,including total phenols,total flavonoids and anthocyanins,and total saponins of the one-year-old P.notoginseng plants under supplemental UV-B stress in fields.[Method] The one-year-old plants were irradiated by UV-B in field for 1 min per day,and the plants under the UV-B lamp were regarded as a circle center,achieving an annular leaf-sampling.The photosynthetic pigment,phenols and total saponins of the leaves were determined spectrophotometrically.[Result] With the increase of sampling radius,the supplemental UV-B intensity decreased significantly,the contents of chlorophyll （Chl） a,Chl b,Chl （a＋b）,carotenoid （Car） and total photosynthetic pigment （Chl＋Car） of the leaves increased extremely significantly,the Chl a/b and total phenol content （TPC） decreased extremely significantly,but the Chl （a＋b）/Car changes were not significant.The contents of total flavonoids,anthocyanins and saponins all increased due to the increasing of UV-B,displaying dose effects.The UV-B intensity was positively correlated with the Chl a/b,and negatively with the Chl a,Chl b,Chl （a＋ b）,Car and （Chl＋Car） contents; and the two of TPC,total flavonoid content （TFC）,total anthocyanin content （TAC） and total saponin content （TSC） were positively correlated,all reaching extremely significant level.The UV-B intensity was positively and significantly correlated with the total flavonoid content （TFC）,negatively and significantly with the Chl （a＋b）/Car,and positively and insignificantly with the TPC,TAC and TSC.[Conclusion] For one-year-old plants of P.notoginseng,UV-B can decrease the contents of the Chl a,Chl b,Chl （a＋b）,Car and （Chl＋Car） and increase the Chl a/b and TPC,and,furthermore,induce the increases of the TFC,TAC and TSC in a dose-dependent manner.However,UV-B can hardly change the Chl （a＋b）/Car.The supplemental UV-B of well
UV-B辐射对植物的形态建成有着重要的作用,并且随着地表UV-B辐射的加剧也必将对作物产生影响。本研究通过测定株高、节距、叶面积和根冠比等几个重要的形态指标,分析在增强UV-B辐射条件下马铃薯不同品种的形态响应差异,为综合评价UV-B辐射对马铃薯的影响和耐受性品种选育奠定基础。试验以4个普通和3个彩色马铃薯品种(系)为材料,采用自然光照(CK)和2个增强的UV-B辐射[2.5 k J?m-2?d-1(T1)、5.0 k J?m-2?d-1(T2)]进行处理,分别于处理15 d、30 d和45 d时测定株高、节距、叶面积和比叶重,收获时测定根冠比。最后得出几个形态指标的响应指数(RI),并以累积胁迫响应指数(CSRI)对试验品种的耐受性做出评价。结果显示:在增强UV-B辐射处理后,多数品种呈现株高降低、节距缩短、叶面积减小、比叶重增加的一致性变化趋势,且随着处理强度的增加和时间延长,处理与对照间的差异愈加显著。各品种对UV-B辐射的形态响应存在显著差异,普通品种的株高、节距、叶面积受UV-B辐射抑制较为明显,地上部分的生物量减幅较大,如‘合作88’地上部分(鲜重)RI值在T1、T2处理下分别为-60.28、-70.44,‘丽薯6号’则为-58.61、-66.44;彩色品种受到的影响较小,‘转心乌’地上部分RI值分别为107.75、21.4,‘21-1’为41.49、-45.72。随着地上生物量增长受到显著抑制,地下部分特别是根系也受到明显的影响,普通与彩色品种间根系RI值的变化规律与地上部分一致。由于地上部分生物量的减幅更显著,各品种(系)的根冠比均比对照增大(T2)。T1、T2处理下5个品种的CSRI值显示‘合作88’(-133.35、-240.85)与‘丽薯6号’(-104.09、-160.2)在增强的UV-B辐射处理下形态特征受到了显著的抑制;彩色品种(系)‘转心乌’(275.97、51.26)、‘21-1’(96.8、-142.17)比普通品种‘合作88’、‘丽薯6号’受到的影响相对较小,显示具有一定
