Using microbore HPLC and electrochemical detection, A microdialysis HPLC assay system was built. After investigating the linear range, detection limit and reproducibility, this method was used to measure the concentrations of DA and monoamine metabolites in striatal microdialysate with 5 min temporal resolution. The detection limit is less than 5 nmol/L. According to the statistical analysis, the result is stable and accurate. It is very helpful to the physiological, pathological and pharmacological research.
目的:本实验利用微透析技术研究椎基底动脉缺血动物模型前庭神经内侧核5-羟色胺(5-HT)的变化,探讨5-HT在后循环缺血眩晕发生中的作用。方法:纯白红目豚鼠12只,采用穿线法阻断基底动脉制作椎基底动脉供血不足动物模型。采用自身对照法进行研究,利用微透析技术动态监测12只豚鼠在椎基底动脉供血障碍前、后前庭神经内侧核5-HT的变化。结果:豚鼠椎基底动脉缺血前前庭内侧核5-HT基础水平为(22.5±1.6)fmol/10μl,椎基底动脉缺血显著地引起前庭内侧核5-HT水平的升高,在10 min后升到最高,达到基础值的(195±19)%。然后略有下降,在30 min 5-HT水平降至基础值的(155±24)%,缺血后30 min内各测试点与缺血前比较差异有显著性(P<0.05)。结论:前庭神经内侧核5-HT的显著性升高可能与椎基底动脉短暂缺血性眩晕的产生有关。
