[Objective] In order to provide firsthand information for the development of fluorescent histology,the paper studied the fluorescent features of ovarioles and germline cysts of fruit fly after common fixation and staining. [Method] With ovaries of Drosophila melanogaster as materials,after fixation with Bouin＇s fluid,the ovaries were sectioned and stained with HE,haematoxylin or eosin,respectively. The specimens were observed and photographed under fluorescent microscope in bright field,and fluorescent fields after excitation with green,blue and UV light,respectively. [Result] After staining by three methods,germ cells and somatic cells emitted different colors of fluorescence after excitation by different lights; lipids,nucleic acids and proteins in cells could also emit their special fluorescence. [Conclusion] Conventional dyes could give different fluorescence characteristics to germ cells and somatic cells,which can also give special fluorescence characteristics to different cellular components. Thus,the fluorescence histology will provide broad prospect for more convenient study on different cell types and cellular components.
