血红素加氧酶-1(Heme oxygenase-1,HO-1)是血红素代谢的限速酶,也称为热休克蛋白32(Heat shock protein 32,HSP32),能被多种刺激因素诱导表达,具有细胞保护作用。研究发现病毒感染可调控HO-1的表达,HO-1的上调表达也可对抗病毒的感染,进而发挥其对细胞的保护作用。尽管HO-1与病毒相互作用的分子机制尚不清楚,但是HO-1与病毒感染的相关性研究已取得了一些成果,可为病毒性疾病的治疗提供新的方法。从病毒感染对HO-1表达的影响和HO-1的表达对病毒感染的影响两方面阐述了HO-1与病毒相互作用的研究进展。
The cultivation of redclaw crayfish(Cherax quadricarinatus) is developing in recent years.The disease of Cherax quadricarinatus was one of the major factors in its culture and even caused redclaw crayfish to die.The viral disease was found in polyculture of redclaw crayfish with Penaeus vannamei.Haemolymph and homogenated gills from diseased Cherax quadricarinatus was filtered and injected to the healthy Cherax quadricarinatus and all injected animals died within 3-5 days,while animals injected with other tissues including hepatopancrease and stomach were still alive.Examination by electron microscopy showed that there were a number of bacilliform viral particles in the nuclei of haemolymph and gill tissues.The virions varied in(225.6-280.5) nm×(73.2-176.8) nm.The detection of the gills and haemolymph by nucleic acid probe and PCR proved that the virus from Cherax quadricarinatus was similar to WSSV.The BLAST results of the sequence showed that it has 100% homology with respect to sequence of AF332093.The sequence reported in this paper has been deposited in the GenBank database(accession number EF078890).The results indicated that this virus belongs to WSSV Chinese.The C.quadricarinatus bacilliform virus could be detected by WSSV nucleic acid probe and PCR.
