Objective: To determine survivin,caspase 3 and fragile histidine traid (FHIT) expression and to analyze its clinical significance in gastric cancer. Methods: Survivin,caspase 3 and FHIT expressions were determined by immunohistochemistry in 32 cases of gastric cancer and 10 cases of normal gastric mucous membrane. At the same time the correlation between survivin,caspase 3 and FHIT expression and the clinical pathological type was analyzed. Results: The positive expression rate of Survivin gene in gastric cancer was 62.5%, but was 0% in normal gastric mucous membrane. The positive expression rate of caspase 3 was 80% in normal gastric mucous membrane and 43.8% in gastric cancer. The positive expression rate of FHIT was 90% in normal gastric mucous membrane and 46.8% in gastric cancer. They all have significant difference with the control but had no significant difference with clinical pathological type. The expression of survivin gene showed negative correlation with caspase 3 and FHIT. Conclusions:Survivin,caspase 3 and FHIT gene participate in the carcinogenesis and progression of gastric cancer. Survivin may inhibit the expression activity of caspase 3 and FHIT gene and prohibit cell apoptosis.
探讨胆内瘘的早期诊断方法。回顾分析2007年1月—2014年6月延安大学附属医院诊治的21例胆内瘘病例资料。术前内镜诊断胆内瘘7例(ER C P诊断5例、结肠镜诊断2例),M R C P诊断2例,消化道造影诊断1例;术中探查诊断胆内瘘11例。术中分离组织损伤门静脉1例,行血管修补治愈。术前拍片、M R C P、C T等影像学检查提示胆道积气,排除胆肠吻合、十二指肠乳头过度松弛及EST手术史,进一步行ER C P、结肠镜检查能提高胆内瘘早期诊断率。
