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李静

作品数:8 被引量:28H指数:2
供职机构:北京大学药学院更多>>
发文基金:国家自然科学基金国家教育部博士点基金国家重点基础研究发展计划更多>>
相关领域:医药卫生更多>>

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Synergistic effect of ethaselen and cisplatin treatment against tumor cell lines被引量:1
2009年
The antitumor activities of ethaselen (BBSKE) in combination with cisplatin (CDDP) in vitro have been investigated in human stomach cancer cell line BGC 823 and human lung cancer cell line PG-BE1. MTT method was used to assess the individual effects of ethaselen and cisplatin and their combined effects on cell proliferation of BGC 823 cells and PG-BE1 cells. Additionally, we used the classic median effect theory to calculate the combination index (CI) of ethaselen and cisplatin with different dose regimes in combination, and compared the effective dosages of cisplatin in individual treatment and combination treatment. When ethaselen and cisplatin were used in combination, a synergistic effect was observed. The most remarkable synergistic effect was observed when the dose ratio of cisplatin to ethaselen was 2:3 in BGC 823 cell line and 1:3 in PG-BE1 cell line. The dose of cisplatin could be decreased markedly in combination group to reach the same inhibitory effect, and this effect was gradually raised with the increase of the concentration of drugs.
李静傅佳凝谭强曾慧慧
乙烷硒啉与顺铂或氟尿嘧啶联合应用对胃癌BGC-823的治疗作用被引量:1
2008年
目的观察乙烷硒啉与顺铂或氟尿嘧啶联合应用对胃癌BGC-823的体内、外抗肿瘤作用。方法MTT法测定5、10、20、40μmol·L^(-1)乙烷硒啉单药及固定其浓度为5μmol·L^(-1)与顺铂或氟尿嘧啶联合时对BGC-823的生长抑制作用。建立裸鼠移植瘤模型,采用乙烷硒啉、顺铂、氟尿嘧啶单药及乙烷硒啉与后两者联合的不同给药方案,观察抗肿瘤作用。结果乙烷硒啉对BGC-823有明显增殖抑制作用,24、48、72h的IC_(50)值分别是30.23、19.70和11.67μmol·L^(-1),联合后效果明显增强(P<0.05)。体内实验,联合组肿瘤增长率明显降低,与单药组有非常显著差异(P<0.01)。结论乙烷硒啉单药表现明显抗肿瘤作用,与顺铂或氟尿嘧啶联合后可以增效。
李静方家椿曾慧慧
关键词:顺铂氟尿嘧啶胃肿瘤乙烷硒啉
金龙胆草总苷对大鼠幽门结扎胃溃疡的防治作用及其机制
目的:研究金龙胆草总苷(Conyzablini-isaponin,CBS)对大鼠实验性胃溃疡的防治作用及其作用机制。方法:采用幽门结扎大鼠(Shay大鼠)胃溃疡模型,预防口服给予CBS,测定胃液、胃酸的分泌量,胃粘膜组织...
马俊江李静苏艳芳郑俊华
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Avalidated HPLC method for the determination of cefazolin sodium level in rabbit synovial fluid被引量:1
2014年
A sensitive and specific high performance liquid chromatography method was established for measuring cefazolin sodium level in rabbit synovial fluid. Acetonitrile was used to precipitate proteins and antipyrine was used as internal standard. Samples were analyzed on a Dionex Ultimate U3000 HPLC system equipped with Phenomenex Luna C18 column (150 mmx4.60 mm, 5 Bm, 100 A). The mobile phase consisted of acetonitrile and 0.1% formic acid in water. The flow rate was 1 mL/min. The detection wavelength was set at 272 nm. The column temperature was maintained at 25 ℃. The calibration curve was linear in the range of 1.0-100.0 μg/mL (r2 = 0.9999). The limit of detection (LOD, S/N - 3) was 0.07 μg/mL. The limit of quantitation (LOD, S/N = 10) was 0.22 pg/mL. The recovery of cefazolin sodium (low, medium and high) was 124.6%, 117.8%, and 100.6% (RSD% - 1.9%, 4.0%, 1.1%, n = 5), respectively. The intra-day and inter-day precision values were in the range of 0.5%-2.7%. The method was simple, sensitive and reliable. It can be used for the quantitative determination of cefazolin sodium in rabbit synovial fluid.
李静林微微李玳敖英芳杨海松凌笑梅
关键词:HPLC
影响中药三七HPLC指纹谱的若干因素被引量:14
2004年
目的 :考察三七药材的不同干燥方法、药材干燥程度和保存时间等因素对三七HPLC指纹图谱的影响。方法 :采用三七HPLC指纹谱的方法 [Luna 5 μC18(2 5 0× 4 6mm) ,乙腈 水梯度洗脱 ,流速 1ml/min ,检测波长 2 0 0nm]来分析运用不同干燥方法得到的三七样品以及不同干燥程度和不同保存时间的三七样品 ,揭示这些因素对三七HPLC指纹谱的影响。结果 :①在不同干燥方法下 ,有的组分受到干燥温度和时间的影响较大 ,有的组分受到药材状态的影响较大 ,有些组分同时受到上述两种因素的影响。② 15号色谱峰为新鲜药材和干燥过程中的药材的特征峰 ,并且其变化是可逆的。③在保存过程中 ,10号和 12号色谱峰的峰面积在保存过程中持续上升 ,其余多数色谱峰的峰面积则随保存时间的延长而逐渐降低 ;不同状态的三七药材 ,粉末药材变化幅度较大 ,而整主根药材中组分的变化幅度较小。结论 :干燥方法。
李静王璇马付勇贾秀虹蔡少青梁鑫淼小松かっ子
关键词:中药HPLC指纹谱
A high-performance liquid chromatography with fluorescence detection method for the simultaneous quantitation of monoamine neurotransmitters and their metabolites in subregions of rat brain被引量:2
2015年
Abstract: In the presem study, we simultaneously quantified the levels of monoamine neurotransmitters (MANTs) and their metabolites (levodopa, norepinephrine, epinephrine, dopamine, 5-HT, 3,4-dihydroxyphenylacetic acid, homovanillic acid and 5-hydroxyindole-3-acetic acid) in different brain subregions of rats using a newly developed simple, sensitive and selective high-performance liquid chromatography with fluorescence detection (HPLC-FLD) method. In this new HPLC-FLD method, analytes were directly extracted and separated without deriveatization step within 20 min. The FLD wavelength was set at 280 nm and 330 nm for excitation and emission, respectively. The analytes were separated on an Agilent Eclipse Plus Cls column (4.6 mm×150 mm, 5.0 μm) equipped with an Agilent XDB-C18 security guard column (4.6 mm×12.5 mm, 5.0 lam), and the column temperature was maintained at 35 ℃. The mobile phase for elution was isocratic. The mobile phase consisted of citric acid buffer (50 mmol/L citric acid, 50 mmol/L sodium acetate, 0.5 mmol/L octane sulfonic acid sodium salt, 0.5 mmol/L Na2EDTA and 5 mmol/L triethylamine, pH 3.8) and methanol (90:10, v/v) at a flow rate of 1.0 mL/min. The detection limit (DL) was 0.9-23 nM for all the MANTs and their metabolites with a sample volume of 50 μL. The method was shown to be highly reproducible in terms of peak area (intraday, 0.08%-1.85% RSD, n = 5). The simultaneous measurement of these MANTs and their metabolites improved our understanding of the neurochemistry in the central nervous system (CNS) in relation to different addictive drugs (methamphetamine, heroin and their mixture) in drug-addicted rat models.
徐鹏白燕平杨海松李静卢炜凌笑梅
小鼠H22肝癌皮下移植瘤的生长与血硫氧还蛋白还原酶活性的相关性被引量:9
2009年
背景与目的:研究表明硫氧还蛋白还原酶(thioredoxin reductase,TrxR)在肿瘤组织中的表达较正常组织中明显增多且活性升高,但关于肿瘤生长与血TrxR活性的关系报道却很少。本实验的目的是研究小鼠H22肝癌皮下移植瘤的生长与血TrxR活性的关系。方法:建立H22肝癌昆明小鼠皮下移植瘤动物模型,分为5组:空白组不接种肿瘤细胞,模型组接种肿瘤细胞,这两组小鼠均给予5‰羧甲基纤维素钠灌胃作为对照;乙烷硒啉低、中、高剂量组均接种肿瘤细胞,并分别灌胃给予36mg/kg、72mg/kg、216mg/kg乙烷硒啉。于肿瘤接种前、肿瘤生长至100mm3给药前、给药第1天、给药第4天及给药第10天5个时间点行眼眶取血,二硫代双硝基苯甲酸(dithio-bis-nitrobenzoic acid,DTNB)法测定血浆TrxR活性。处死小鼠剥离皮下肿瘤,DTNB法测定肿瘤组织TrxR活性。结果:给药结束时低、中、高剂量乙烷硒啉组小鼠的抑瘤率分别达到59.5%、74.3%、74.9%。空白组小鼠血TrxR活性稳定;接种肿瘤后小鼠血TrxR活性随肿瘤体积增大而增加,呈明显正相关;给药结束时,低、中、高剂量乙烷硒啉组小鼠血TrxR活性抑制率分别为59.2%、68.4%、68.9%,而肿瘤组织中TrxR活性抑制率分别为15.3%、25.8%、29.8%。结论:H22肝癌小鼠血TrxR活性与其肿瘤生长呈正相关;不同剂量的TrxR抑制剂乙烷硒啉干预后,血TrxR活性能够被不同程度地抑制,与肿瘤生长的抑制呈一致性。
谭强任晓远李静王芳邓声菊曾慧慧
关键词:硫氧还蛋白还原酶乙烷硒啉移植瘤小鼠
中药三七乙酰化前后化学组成的检测和比较研究(英文)
2004年
目的:研究二七提取液衍生化(本文采用了乙酰化)和蒸发光散射检测器(evaporative light-scatteringdetector,ELSD)在中药三七组分分析和鉴定中的应用。方法:采用乙腈-水梯度洗脱、紫外检测的方法,比较乙酰化前后的三七样品提取液的高效液相色谱(high pedbrmance liqmd chromatography,HPLC)图谱差异;分别采用乙腈-水(体积比30∶70)和乙腈-水(体积比85∶15)作为流动相,比较用 ELSD 和紫外检测器时三七样品提取液的HPLC 图谱差异。结果:在三七提取液乙酰化后,可以观察到在原样品色谱图中较早洗脱出的组分的变化,其中某些组分衍生物的保留时间大大延长。分别用 ELSD 检测和紫外检测可以得到不同的色谱图,用 ELSD 检测方法可以得到几种不同变化方式的峰群,从而提供了更多的三七提取液化学组成的信息。结论:三七提取液乙酰化后,其中一些亲水组分的极性大大降低,那些在反相色谱中较早流出、较难分离的组分经衍生化后出峰状况得到改善,ELSD 还可以检测到有药理作用但因没有紫外吸收较难检测的糖类。
李静石玉杰徐秉玖
关键词:中药ELSD乙酰化HPLC图谱乙腈化学组成
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