From November 2008 to January 2009,a sharp increase of diarrhea in children in Guangdong province appeared,we randomly collected 53 stool specimens from out-patient children with dirrhea in 3 major hospitals (Guangzhou City Children’s Hospital,Shenzhen Baoan District Maternal and Child Health Hospital,First Affiliated Hospital of Shantou University). Rotavirus and calicivirus were screened by ELISA and RT-PCR. We found 29 cases of rotavirus infection with diverse serotypes. Only four cases were identified as calicivirus infection. The result indicated that rotavirus was a major pathogen of this high incidence of diarrhea from November 2008 to January 2009 in Guangdong Province.
Group A rotavirus are the most frequently detected viral agent associated with the acute diarrhea in calves.In order to investigate the situation of rotavirus strains circulating in diary farms,a total of 117 fecal specimens were collected from diarrhea calves under 4 weeks-age on Yinluo diary farm in Daqing region in China from 2008 to 2009.Ten specimens were detected to be positive by a Rotavirus Group A Diagnostic Kit,which confirmed that the rotavirus was important viral agent associated with diarrhea in this diary farm.Based on the new classification system,G10P[11] genotype was determined in rotavirus positive samples.Sequence and Phylogenetic analysis indicated DQ-75 strain was introduced into our country with imported bovine.
