[Objective] This study aimed to explore the heterogeneity of chlorophyll flu- orescence of Phyllostachys edulis cv. Pachyloen leaves. [Method] Using the chloro- phyll fluorescence system and fluorescence imaging system in the Mini-IMAGING- PAM, the chlorophyll fluorescence of Phyllostachys edulis cv. Pachyloen leaves were measured and the fluorescence parameters were calculated. [Result] The homo- geneities of the maximal quantum yield of PS II （Fv/Fm） and the leaf absorptivity （Abs） were higher, with CVs （Coefficient of Variation） of 1.58%-1.68% and 1.75%- 2.12% respectively, while the heterogeneities of the actual quantum yield （Y）, non- photochemical quenching （NPQ/4）, photochemical quenching（qP） and relative photo- synthetic rate （PS/50） were higher, with CVs of 9.60%-14.23%, 10.23%-13.02%, 11.92%-13.02% and 11.15%-17.74% respectively. The trends of heterogeneity change in Y, qP and PSI50 were basically the same with transversely higher het- erogeneity at two sides and lower heterogeneity in the middle, namely with larger CVs at the edges of leaves and smaller ones around the midrib. Longitudinally, the coefficients of variation of Y, qP and PS/50 decreased gradually （from top to bot- tom）, which indicated that the heterogeneity declined from the leaf tip to the base. The trends of heterogeneity change in PS/50 and NPQ/4 were opposite. [Conclusion] The fluorescence parameters of Phyllostachys edulis cv. Pachyloen leaves revealed different heterogeneity.
