[Objective] This study aimed to screen a set of SSR core primers suitable for purity identification of pepper （Capsicum） hybrids. [Method] DNA fingerprint of 100 pepper hybrids was analyzed using 17 SSR primers. [Result] According to the polymorphism and heterozygosity, Hpms1-214, Es395 and Hpmsl-5 were determined as three preferred core primers for purity identification of pepper hybrids. By using these three preferred core primers, 97 pepper hybrids （accounting for 97%） had heterozygous band pattern with at least one primer. Es330, Es363, Epms923, Es120 and Es64 were determined as candidate core primers for purity identification of pepper hybrids. Specific primers of 14 varieties were obtained, which could be used to further screen parent-complementary primers of each pepper hybrid. [Con- clusion] This study laid the foundation for constructing standard DNA fingerprints for purity identification of pepper hybrids.
By field experiments with Uniform Design, the effects of planting density and nitrogen rate on three varieties （Yunrui No.8, Yunrui No.6 and Yunrui No.88） grain yield under different ecological conditions were studied in Yunnan Province. The results showed that the grain yields were different among the five experiment locations, when increasing of planting density, it did not affected the plant height, ear height and ear factor, with the planting density increased, stem diameter, leaf width, ear length, number of grains per row and 1 000-grain weight decreased sig- nificantly, but the grain yield of the third varieties increased. High planting density had a negative impact on ear traits, but the grain yield increased by high-density compensate for the adverse effects.Control space and time of fertilizer application can reach the highest yield under less Nitrogen fertilizer Yunrui 88 had the charec- teristics of wide range of adaptability and the density-tolerance, high-yielding poten- tial, which was favorable in planting on large areas.
