Six compounds were isolated from ethanol extract of fresh fruit peel of Fortunella margarita(Lour.) Swingle by column chromatography.By means of MS and NMR technique,the six compounds are identified as follows: fortunellin(Ⅰ),phloretin-3′,5′-di-C-β-glucopyranoside(Ⅱ),rutin(Ⅲ),4′-methoxy vitexin-2″-O-α-L-rhamnopyranoside(Ⅳ),rhoifolin(Ⅴ) and β-daucosterol(Ⅵ),in which contents of compound Ⅰ and Ⅱ are the highest and compound Ⅴ is obtained from fruit peel of F.margarita at the first time.