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朱元军

作品数:24 被引量:36H指数:3
供职机构:北京大学药学院分子与细胞药理学系更多>>
发文基金:国家自然科学基金北京市自然科学基金国家科技支撑计划更多>>
相关领域:医药卫生理学生物学更多>>

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24 条 记 录,以下是 1-10
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具有溶栓和脑保护作用的融合蛋白TBN及其编码基因和应用
具有溶栓和脑保护作用的融合蛋白TBN及其编码基因和应用。本发明公开了一种具有溶栓和神经保护双重作用的融合蛋白及其编码基因和应用。所述融合蛋白,其氨基酸序列如SEQ ID NO.2所示。此外,本发明还公开了编码该融合蛋白的...
王银叶陈欢朱元军刘晓岩郑丹萍
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研磨法制备金属有机框架材料的新进展被引量:1
2022年
金属有机框架材料是由金属离子/团簇和具有一定刚性结构的有机配体通过配位键连接而成的多孔晶体材料,具有多孔、比表面积大、结构多样、表面易修饰等特点,在能源、化工、医药领域具有广泛的应用。机械化学合成法是指通过机械能诱导化学反应的方法,由于其绿色环保、耗时短、效率高、应用范围广、副反应少的特点近年来受到广泛关注,在制备金属有机框架材料方面同样表现出显著的优势。研磨法是机械化学合成法中重要的一种。为了解生物金属骨架材料的机械化学法合成概况及最新进展,本文介绍了研磨法制备金属有机框架材料的经典案例,尤其着重介绍了应用于医药领域的金属有机框架材料的合成,研究进展表明研磨法是一种绿色高效的合成方法,为金属有机框架材料在医药领域的广泛应用提供了可能,具有良好的发展前景。
张珂屈小虎朱元军林建英赵志换樊惠玲
关键词:机械化学法配合物药物递送
Anti-thrombotic effect of W007B,a newly synthesized compound,in vitro and in vivo被引量:2
2014年
In the present study, we investigated anti-thrombotic effects of W007B, a water-soluble derivative of honokiol, with different models both in vitro and in vivo. Rat platelet aggregation was induced by adenosine diphosphate (ADP), thrombin, arachidonic acid (AA) and collagen in vitro. The anti-thrombotic effects were evaluated with the arterio-venous shunt model, electrode-stimulated carotid thrombosis model in rats and ADP-induced acute pulmonary embolic model in mice. The bleeding time in vivo was examined with tail incision in mice. W007B inhibited ADP-, thrombin-, collagen- and AA-induced platelet aggregation in a concentration-dependent manner, with an ICs0 value of 899.5 μM, 212.9 μM, 266.0 μM and 52.5 μM, respectively. In vivo, W007B (2-10 mg/kg, ig) significantly reduced the thrombus weight in the model of arterio-venous shunt. Besides, W007B could effectively prolong the occlusion time in the electrode-stimulated carotid thrombosis model. Moreover, in the ADP-induced acute pulmonary embolism model in mice, 2.8-14 mg/kg of W007B significantly reduced the death of mice. In conclusion, W007B is effective on platelet aggregation, and it is most sensitive on AA-induced aggregation. W007B has potent anti-thrombotic effect on different arterial thrombosis models. It may be an orally active candidate of anti-thrombotic agents.
胡琨刘晓岩朱元军张精亮刘晔王银叶
关键词:ANTIPLATELETANTITHROMBOSIS
Design, preparation and activity evaluation of novel recombinant thrombolytic proteins
2017年
Recombinant tissue plasminogen activator (rPA) has been used as a thrombolytic agent. However, considerable improvements have been done to prolong its plasma half-life (tl/2) and reduce its side effects, such as intracranial hemorrhage. Based on these improvements, a mutant ofrPA, mrPA, was designed by mutating its PAI-1 binding site to extend its tl/2. Furthermore, a fusion protein conjugating mrPA with NR3 was designed, which was a rAcAp5 mutant with a platelet GPIIb/IIIa-binding RGD motif, to enhance the ability of targeted-thrombus and thrombolysis. The synthesized DNA sequences coding the two proteins were amplified by PCR, cloned into pET30a to construct recombinant plasmids pET30a-mrPA and pET30a-mrPA-NR3, and transformed into E. coli BL21 (DE3). The two proteins were expressed in inclusion bodies induced by isopropy113-D-1-thiogalactopyranoside. After purified to qualified purity using one-step Ni affinity chromatography, the denatured proteins were refolded by dialysis. Their thrombolytic effects in vitro and in vivo were evaluated. In vitro 3.5 and 7 pmol/L of mrPA significantly reduced thrombus weight; 1.75, 3.5 and 7 ~tmol/L of mrPA-NR3 also significantly reduced the thrombus weight, and mrPA-NR3 displayed stronger thrombolytic effects than mrPA at 7 lamol/L. In vivo both mrPA and mrPA-NR3 showed significantly thrombolytic effect at 60-240 ktmol/kg in thrombolytic model of inferior vena cava. Importantly, mrPA-NR3 exhibited more potent thrombolytic effect than both mrPA and rhM-tPA (positive control) at 240 p.mol/kg. In addition, these two novel proteins did not increase bleeding time while they exerted thrombolytic effect. In conclusion, we engineered two novel proteins and proved that fusion protein had better thrombolytic effect than non-fusion protein, and the results suggest that dual thrombolytic mechanism or thrombus-target potentiated the thrombolytic effect ofrPA and alleviated hemorrhage side reaction. This study may shed light on the development of novel thrombolytic agents
胡桂珍刘晓岩朱元军王银叶
防御素抗HIV作用被引量:16
2006年
防御素(defensins)是小分子阳离子抗菌肽,它们既是先天性免疫的效应分子,也参与获得性免疫的调节。大量的研究发现防御素能有效抑制HIV,本文将扼要综述防御素的结构和来源分布,并重点讨论防御素抗HIV的效能及其机制。
朱元军李明远
关键词:防御素HIV先天性免疫
门冬氨酸钾镁对离体灌流心脏缺氧的保护作用被引量:6
2006年
目的探讨门冬氨酸钾镁对离体心脏缺氧的保护作用,及2种不同制剂组成方式的门冬氨酸钾镁注射液的效能比较。方法将60只SD大鼠取心脏行Langendorff离体心脏灌流,依照灌流液中钾、镁组成的不同分为A制剂组(门冬氨酸钾+门冬氨酸镁)、B制剂组(天门冬氨酸+氧化镁+氢氧化钾)及对照组(KCl+MgSO4)3组,每组各20只动物,3组的K+、Mg2+等剂量。记录离体大鼠心脏在缺氧条件下的HR、左室压力变化及心脏跳动持续时间。结果A制剂组中离体心脏在缺氧后HR及左室压力降低速度缓慢,心脏跳动持续时间达(273.9±65.2)min,与B制剂组(174.7±43.6)min及对照组(121.8±32.9)min相比明显延长(P<0.01)。结论门冬氨酸钾镁液对离体缺氧心脏有保护作用,明显延长缺氧状态下心脏的跳动时间,但制剂B比制剂A的抗缺氧效能差。
朱元军苏亮张奎王树人
关键词:门冬氨酸钾镁缺氧
mBD2与HA1融合基因真核表达载体的构建及其免疫保护性研究
甲型流感病毒曾在世界上多次发生大规模的流感暴发流行,目前对流感尚无特效药物进行治疗,接种流感疫苗是易感者预防流感的最佳选择,核酸疫苗是当前流感疫苗研究的方向与热点。流感病毒血凝素(hemagglutinin.HA)是流感...
朱元军
关键词:血凝素Β防御素融合基因DNA疫苗真核表达免疫保护
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rAcAP5: high-yield strain screening, expression, purification and thrombolytic effect evaluation in rat embolic middle cerebral artery occlusion model
2014年
Recombinant ancylostoma caninum anticoagulant peptide-5 (rAcAP5) has been reported to inhibit thrombin-activatable fibrinolysis inhibitor (TAFIa) activity and have thrombolytic effect. The present study was to screen a strain expressing high-yield of rAcAP5 and to assess its thrombolytic effect on embolic middle cerebral artery occlusion (MCAO) model in rats. Codons encoding for AcAP5 were optimized. Six expression plasmids and eleven E. coli strains with different characteristics were used, a total of 66 recombinant expression strains were generated and the one with the highest yield was selected to express rAcAP5, which was purified through anion- and cation-exchange chromatography. The purity of rAcAP5 and its molecular weight were determined by HPLC and mass spectrometry, respectively. The thrombolytic effect of rAcAP5 was evaluated on embolic MCAO model in rats; regional cerebral blood flow (rCBF) was monitored with a Laser-Doppler flowmetry to test the occlusion and recanalization of MCA. The highest yield recombinant strain was C2566H/pTYB 1-rAcAP5. AcAP5 (28 mg) with 90% of purity was obtained from 1 L of cell culture. In rat embolic MCAO model, vehicle (normal saline) treatment did not change the rCBF, while treatment with rAcAP5 (50-200 μg/kg, i.v.) increased the rCBF in a dose-dependent manner. In conclusion, we prepared and characterized the rAcAP5 peptide and revealed its thrombolytic effect in embolic MCAO model and our results suggested that this peptide had the potential to be used as a thrombolytic agent.
朱亚楠朱元军卜琦鑫刘晓岩王银叶
关键词:OVER-EXPRESSIONPURIFICATIONTHROMBOLYSIS
驱动蛋白超家族在多种疾病的发生和发展中的作用被引量:4
2017年
细胞内物质运输是细胞发挥正常生物学功能的基础.驱动蛋白可作为运送细胞内物质的载体,通过与不同的骨架蛋白结合以识别不同的分子货物,从而参与这些分子货物下游的生物学效应.没有运输活性的驱动蛋白也可以通过其自身对某些分子信号通路的调节而发挥其功能.大量研究表明,驱动蛋白广泛地参与了多种疾病的发生发展过程,如神经性疾病、代谢性疾病、肾病、癌症等.本文将对近年来诸多关于驱动蛋白与疾病的研究进行综述.
杨文星刘鹤王力朱元军
关键词:驱动蛋白分子马达微管
一种具有靶向和长效功能的重组水蛭素融合蛋白及其编码基因和应用
本发明公开了一种具有靶向和长效功能的重组水蛭素融合蛋白及其编码基因和应用。所述融合蛋白,由“促凝血小板”靶向结合肽、水蛭素,以及白蛋白结合结构域融合组成。该融合蛋白具有前药性质,能体内快速激活、发挥抗血栓形成效应;且和水...
王坚成朱元军韩壶壶
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