[Objective] The aim was to study the supporting raising technology of Castanea mollissima Blume molecular marker-assisted selection to reduce the cost and time for chestnut breeding.[Method] To find the best cutting method,10 treatments were designed according to transverse diameter and vertical diameter of the chestnut including different cutting ratios in 1/2,1/3,1/4 and different directions about cross cutting,vertical cutting,bevel cutting and two side-cutting.The germination rate,seedling survival rate and morphological indicators of the treatments were measured;significant difference among them was also analyzed.[Result] 1/3 bevel cutting was the best,which not only could give high germination rate and survival rate,but also could guarantee high quality of nursery stock,which did not grow so differently with the untreated seedlings.[Conclusion] This way can not only reduce too much cost and time for seedling breeding,but also promote the germination of chestnut in advance,which could do much good to production.
