IIn order to ascertain the suitable machine-transplanted density of Lingliangyoul04 in Hunan, a field experiment was conducted in Mingyue Village of Lukou Town of Changsha County in 2013, and the effects of three machine-transplanted densities (DI: 333 000 holes/hm^2, D2 250 000 holes/hm^2, D3 175 000 holes/hm^2) on yield and yield formation of Lingliangyou104 were studied. The results indicated that the yield of Lingliangyoul04 showed an increase tendency with the increasing of machine-transplanted density; the highest yield of Lingliangyou104 showed in D1 by 9.66 t/hm^2, it increased by 12.2% and 22.4% compared with D2 and D3, respectively. Under high machine-transplanted density, Lingliangyoul04 performed with more effective panicles, large index of leaf area, high probability of intercepting light radiation by canopy, and strong leaf photosynthetic capacity, thus increasing amount of dry matter production and high yield.