AIM To study the changes of (endothelin, ET) in cultured human umbilic vein endothelial cells (HUVEC) with Hcy in different concentration. METHODS HUVEC were collected and cultured to the third generation. Then HUVECs were cultured with Hcy in different concentration (physiological concentration 0.01 mmol·L -1 , pathophysiological concentration 0.5 mmol·L -1 , pharmacological noncytotoxic concentration 2.0 mmol·L -1 , pharmacological cytotoxic concentration 5.0 mmol·L -1 ) for 24 hours. ET in culture medium were examined in 4, 6, 8, 24 hours. RESULTS After HUVECs were exposed to Hcy in different concentration for 24 hours, ET was reduced significantly. CONCLUSION Hcy could destoried the release of ET in cultured HUVEC.
