[Objective] This study was to investigate the climatic factors affecting the regional eco-adaptability of ramie.[Method] Five experimental sites of different eco-types in ramie growing regions in Yangtze Valley were set up to investigate the yield of Zhongzhu No.2,based on which we assessed the adaptability of Zhongzhu No.2 to various eco-regions and further analyzed the climatic factors causing the difference in eco-adaptability.[Result] Ramie yield varied largely among various experimental sites and the yield difference appeared to be well repeatable,with a differential value between the maximum and the minimum reaching 2.3 kg/20 m2.Analysis of the yield data,together with climatic factors in various ecological regions showed that yield of Zhongzhu No.2 was significantly correlated with rainfall,sunshine hours,relative humidity during its growing period.Of the climatic factors,sunshine hours and rainfall can positively promote yield increase,while relative humidity negatively regulate ramie yield.[Conclusion] The climatic indices,including rainfall,sunshine duration and relative humidity are a key factor causing yield difference in ramie among different regions of Yangtze Valley.