[ Objective] The aim was to study the effect of 12C6 + ions beam irradiation to two varieties of sweet sorghum on seed germination and some enzymes activity in seedlings with different doses, and provided a theoretical foundation for sweet sorghum breeding. [ Method] After germination, the germination potential, germination fraction and enzyme activity were detected, respectively. [ Result] The results showed that with the dose increased, the germination potential of sweet sorghum increased first and then decreased, while their germination fraction presented "shoulder like shape" ; the activity of LDH, SOD, CAT and GSH-Px increased first and then decreased with doses, they presented slight differences among different enzymes. [ Conclusion] Low dose radiation could accelerate germination of sweet sorghum seeds and enzyme activity could remain at a relatively high level. Enzyme activity decreased with high doses and the growth of sweet sorghum was inhibited.