[Objective] The aim was to explore effects of different curing techniques on starch contents of tobaccos from different growth parts and with varying maturity degrees in order to reduce starch content of Yunnan tobacco. [Method] Compar- isons were conducted on tobaccos from upper, middle and down parts （immature, premature, mature and excessive mature） by low temperature and low humidity cur- ing technique, moderate temperature and moderate humidity curing technique, mod- erate temperature and high humidity curing technique to measure starch contents before and after curing. [Result] The results showed starch content of tobacco was increasing upon growth part. Low temperature and low humidity curing technique has poor effects on reduction of starch content; moderate temperature and moderate humidity curing technique improves starch content; moderate temperature and high humidity curing technique dramatically reduces starch content of tobaccos. [Conclu- sion] With consideration of tobacco appearance, inner quality and characters, moder- ate temperature and moderate humidity curing technique is recommended for tobac- co production.
采用平板划线分离方法对造纸法再造烟叶涂布液中的主要微生物群进行分离,再根据细菌的菌落形态、菌体形态、生理生化实验和16S r DNA序列分析对菌株进行了鉴定。结果表明,鉴定出引起涂布液腐败的细菌为短小芽孢杆菌、地衣芽孢杆菌和肺炎克雷伯菌,其中肺炎克雷伯菌为主要腐败菌。此外,还考察了山梨酸钾、双乙酸钠、尼泊金酯钠、乙二胺四乙酸二钠和ε-聚赖氨酸等5种防腐剂对肺炎克雷伯菌的抑菌效果。结果表明,5种防腐剂的抑菌效果强弱顺序为ε-聚赖氨酸>乙二胺四乙酸二钠>双乙酸钠>山梨酸钾>尼泊金酯钠,其中ε-聚赖氨酸对肺炎克雷伯菌的最小抑菌质量浓度为0.075 mg/m L。
