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国家自然科学基金(20935005)

作品数:5 被引量:13H指数:3
相关作者:陆金鑫马会民更多>>
相关机构:中国科学院更多>>
发文基金:国家自然科学基金国家重点基础研究发展计划更多>>
相关领域:理学生物学医药卫生农业科学更多>>

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酶反应动力学的毛细管电泳及整体柱色谱研究
<正>在药物研究、生命科学研究等领域中,手性分析及蛋白质的高效快速分离是一个重要的课题。由于氨基酸是重要的手性药物合成前体,而蛋白质是生命体中重要的营养物质,并且氨基酸酶和蛋
齐莉姚春荷乔娟张海枝杨更亮陈义
关键词:整体柱
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A new Cu^(2+)-induced color reaction of a rhodamine derivative N-(3-carboxy)acryloyl rhodamine B hydrazide被引量:3
2011年
A new rhodamine derivative,N-(3-carboxy)acryloyl rhodamine B hydrazide(CARB),has been synthesized,and its unusual spectroscopic reaction with Cu2+ has been investigated.The derivative exhibits a rapid and reversible non-fluorescent absorption upon coordination to Cu2+,which is a rather unusual phenomenon for rhodamine B derivatives.Stoichiometric measurements using the Job's method and the molar ratio method reveal that one CARB molecule combines two Cu2+ ions,and the two Cu2+ ions play different roles:one opens the spirocyclic structure and the other quenches the fluorescence of the xanthene moiety.This reaction mechanism is supported by a comparative study on the model compound N-acryloyl rhodamine B hydrazide as well as by the density functional theory calculations.Furthermore,the absorption response of CARB is highly selective for Cu2+ over other common ions,which implies that CARB may be used as a colorimetric probe for the rapid visual detection of Cu2+.
SUN ChengDongCHEN JianMingMA HuiMinLIU YangZHANG JingHuaLIU QingJun
关键词:COPPER
新型相分离剂及聚合物整体柱的制备与应用研究
合物整体柱具有传质快、渗透性佳、稳定性高和易于化学修饰等优点在生化分离、催化、吸附萃取等领域有着广泛的应用,而制备不同于传统颗粒堆积结构的具有 3D 骨架结构的聚合物整体柱对于进一步提高柱效有着重要的意义.目前,3D 骨...
沈莹齐莉乔娟毛兰群陈义
In vivo electrochemical recording of continuous change of magnesium in medial vestibular nucleus following vertigo induced by ice water vestibular stimulation被引量:2
2013年
Vertigo is one of the most common clinical symptoms. However, the chemical processes involved in the pathological mechanism of vertigo remain to be fully understood. In this study, we investigate the dynamic changes in the magnesium (Mg 2+ ) concentration in medial vestibular nucleus (MVN) of guinea pigs following vertigo induced by vestibular ice water stimulation with an electrochemical detection method consisting of in vivo microdialysis and on-line selective electrochemical detection. Electrochemical detection of Mg 2+ was accomplished based on the current enhancement of Mg 2+ towards the electrocatalytic oxidation of NADH at the electrodes modified with the polymerized film of toluidine blue O (TBO). Selectivity for the on-line electrochemical detection against Ca 2+ was achieved by using ethyleneglcol-bis(2-aminoethylether) tetraacetic acid (EGTA) as the selective masking agent for Ca 2+ . The basal level of the extracellular Mg 2+ in the MVN of guinea pigs was determined to be 759.7 ± 176.2 M(n = 16). Upon ice water irrigation of the left external ear canal, the concentration of Mg 2+ in the MVN decreases significantly, reaches 72 ± 6% (n = 8) of the basal level, and maintains for at least 1000 s. Control experiments reveal that neither warm water irrigation of the external ear canal nor ice water irrigation of the auricle induces the decrease in the concentration of Mg 2+ in the MVN. These results demonstrate that the extracellular Mg 2+ in the MVN decreases significantly following vertigo induced by vestibular ice water stimulation. This demonstration suggests that Mg 2+ might play an important role in the pathological mechanism of vertigo.
XIN YingZHANG ZiPinYU PingMA FuRongMAO LanQun
关键词:MAGNESIUMVERTIGO
3,4-Dinitrobenzamide Functionalized CdTe/ZnTe Quantum Dots as a Nanoprobe for Imaging Glutathione S-Transferase in Living Cells
2013年
A novel fluorescent nanoprobe for glutathione S-transferase (GST) has been developed by incorporating 3,4-dinitrobenzamide (a specific substrate of GST) onto CdTe/ZnTe quantum dots. The probe itself displays a low background signal due to the strong quenching effect of the electron-withdrawing unit of 3,4-dinitrobenzamide on the quantum dots. However, GST can efficiently catalyze the nucleophilic substitution of reduced glutathione on the p-nitro group of the nanoprobe, leading to a large fluorescence enhancement. Most notably, this enhancement shows high selectivity and sensitivity towards GST instead of the other biological substances. With this nanoprobe, a simple fluorescence imaging method for intracellular GST has been established, and its applicability has been successfully demonstrated for imaging GST in different living cells, which reveals that A549 cells express GST about 3 times higher than NIH-3T3 and Hela cells.
Jinxin Lu Yanchao Song Wen Shi Xiaohua Li Huimin Ma
关键词:FLUORESCENCENANOPROBEBIOIMAGING
A water-soluble fluorescence resonance energy transfer probe for hypochlorous acid and its application to cell imaging被引量:3
2011年
A water-soluble fluorescence resonance energy transfer (FRET) probe for hypochlorous acid (HOCl), dansyl rhodamine B piperazinoacetohydrazide, was designed, synthesized and characterized. The dansyl moiety in the probe acted as a FRET donor and the rhodamine moiety acted as a FRET acceptor. The two moieties were connected by a HOCl-cleavable active bond, and cleavage of this linker decreased the FRET efficiency and increased the fluorescence intensity of the donor at 501 nm. The water solubility of the probe was improved compared with other probes by introduction of the cationic rhodamine fluorophore. As a result, the probe could be used to detect HOCl in aqueous biosystems with a linear range of 2-10 mol/L and a detection limit of 80 nmol/L (signal- to-noise = 3). The probe was successfully applied to fluorescence imaging of HOCl in HeLa cells.
JIA Jia MA HuiMin
关键词:荧光共振能量转移次氯酸细胞成像FRET罗丹明B
利用试卤灵类荧光探针检测硝基还原酶及细胞缺氧状态
我们以7-羟基-3H-吩恶嗪-3-酮('resorufin)为荧光母体,5-硝基呋喃作为特异性反应基团,设计合成了一种检测硝基还原酶及细胞缺氧的新型荧光探针,7-[(5-硝基呋喃-2-基)甲氧基]-3H-吩恶嗪-3-酮(...
李照李晓花高兴会马会民
关键词:细胞缺氧荧光探针
温敏、盐敏、pH敏有机聚合物整体介质的研究与应用
<正>整体多孔介质在液相色谱固定相的应用发展近年来引起了人们广泛的研究兴趣。因该介质具有微米级骨架和较大的通孔,所以,更能满足人们对快速、高通量分离的要求,同时,对整体多孔介质表面化学性质的改性有助于将其应用于生物大分子...
齐莉魏晓奕张荣月姚春荷辛培勇乔娟沈莹杨更亮陈义
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基于肾缺血的酶反应及酶抑制剂筛选的CLE-CE研究
n(II)为中心离子,以不同的L-氨基酰胺为手性配体,对氨基酸进行了系统的手性配体交换-毛细管电泳(CLE-CE)技术研究:① 通过分析基于肾缺血酶底物的浓度变化实现了对氨基酸氧化酶活性的研究;② 针对神经损伤性疾病开展...
齐莉张海枝乔娟毛兰群陈义
两亲嵌段聚合物毛细管涂层及其在芳香胺CEC分离中的应用
以苯乙烯和马来酸酐为单体,采用RAFT方法合成了一种嵌段聚合物,发现:将该两亲嵌段聚合物作为毛细管涂层,能够起到表面活性剂的作用,进一步将其应用于Baylis-Hillman反应中的原料和产物以及芳香胺的CEC分离中。
乔娟齐莉马会民刘虎威杨更亮陈义
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