A novel alkynyl substituted N-aryl-1,8-naphthalimide II （C20H11NO2） was synthesized for the discovery and evaluation of new fluorescence probes, and was characterized by X-ray crystal diffraction. It crystallizes in monoclinic, space group P21/n with a = 15.898（3）, b = 5.0102（10）, c = 17.962（4）A, β = 92.97（3）°, V = 1428.7（5） A^3, Z = 4, Mr = 297.30, Dc = 1.382 g/cm^3, F（000） = 616,/J = 0.090 mm^-1, S = 1.011, the final R = 0.0542 and wR = 0.1204 for 1669 observed reflections with I 〉 2σ（I） and 212 variable parameters. Hydrogen-bounding and n-stacking interactions were discussed. The influence of acetylene group on the fluorescence properties was also investigated. In comparison with N-phenyl-1,8-naphthalimide I （C18H11NO2）, few new structure characters and fluorescence properties of the title compound have been found.
