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吉林省教育厅资助项目(2011)

作品数:5 被引量:11H指数:2
相关作者:蔡立格王光野魏健盖玉红曲红岩更多>>
相关机构:东北师范大学吉林农业大学吉林省实验中学更多>>
发文基金:吉林省教育厅资助项目国家自然科学基金博士科研启动基金更多>>
相关领域:农业科学生物学更多>>

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Coprinopsis cinerea漆酶基因的克隆及其在毕赤酵母中的表达被引量:3
2012年
通过反转录反应从Coprinopsis cinerea okayama 7#130中克隆得到laccase 1,应用SignalP 3.0 Server软件分析其氨基酸序列后,设计不含信号肽序列的新引物扩增得到漆酶基因,构建重组酵母表达载体pPIC9K-lac1,电击转化毕赤酵母GS1115,并用甲醇诱导表达,之后研究重组酶的酶学性质。克隆得到的laccase 1碱基数为1 593 bp,编码530个氨基酸,其中信号肽包含18个氨基酸。SDS-聚丙烯酰胺凝胶电泳(SDS-PAGE)显示重组蛋白大小约为65 kDa。酶学性质研究表明,该酶最适反应温度为45℃,最适pH值为4.3。成功分泌表达的漆酶活力达到1.108 U/mL。
华欣春陈丽丽毕云枫沈明浩
关键词:漆酶基因毕赤酵母分泌表达
The Research of Ecological Nutritional Organs of Invasive Plants Structural Characteristics
2011年
[Objective] The aim of this paper was to discuss the adaptability of ecological nutritional organs of invasive plants.[Method] By dint of high-definition display of plant tissue crystal method and paraffin section method,the vegetative organs root,stem and leaf cross-section of the organizational structure of Asteraceae Ambrosia trifida L.ragweed(Ambrosia trifida L.)in the suburbs of Changchun City in Jilin Province were inspected.[Result] The structural basis for ecological invasive plant Ambrosia trifida L.vegetative organs was mainly through strengthening the roots of water absorption and transporting function,increasing the water storage function of leaf and stem,reducing the water absorption of stem and leaf for plants to promote water absorption and to enhance the assimilation of leaves to achieve a variety of complex environmental conditions in the rapid growth and development in order to achieve an ecological invasion.[Conclusion] The study provided reference for the biological fundamental research of Ambrosia trifida L.
魏健盖玉红蔡立格曲红岩王英伟王光野
关键词:CRYSTAL
生态入侵植物的营养器官结构特性研究
2011年
[目的]探讨生态入侵植物营养器官对环境的适应特性。[方法]采用高清显示植物组织晶体方法和石蜡切片法,对吉林省长春市郊区环境中的菊科豚草属三裂叶豚草(Ambrosia trifidaL.)的营养器官根、茎和叶横切面的组织结构进行了观察。[结果]生态入侵植物三裂叶豚草营养器官适合生态入侵的结构基础主要是通过加强根系的水分吸收和输导功能、提高茎叶的水分贮藏功能、降低茎叶水势促进植物对水分的吸收以及加强叶片的同化作用来实现在各种复杂环境条件下迅速生长发育,从而实现有效的生态入侵。[结论]为三裂叶豚草生态入侵的生物基础研究提供了参考。
魏健盖玉红蔡立格曲红岩王英伟王光野
关键词:三裂叶豚草晶体生态入侵
Comparison of the Evolvement Structure of Chenopodium album L.under the Stress of Different Saline Environments
2011年
[Objective] The aim of this study was to reveal the evolvement structures,especially the crystal characteristics of Chenopodium album L.under saline stress,so as to providing the first-hand data for utilizing biological techniques to control saline environment.[Method] Employing high definition display method of plant crystal structure and paraffin-section method,we performed a comparative study on the evolvement structures of C.album growing in high salinity areas in the coast of Egyptian Red Sea and common salinity areas in the grasslands in Changling County of Jilin Province.[Result] The regionally distributed crystal and the developed assimilating tissue of C.album are the key structural characteristics to antagonize the saline stress during the evolving process.Stem cortex of C.album growing in both the high salinity areas in coast of Egyptian Red Sea and common salinity areas in the grasslands in Changling County of Jilin Province has similar discontinuous crystal rings.Assimilating tissue in C.album growing in high salinity areas is highly developed than that in common salinity environment.Comparative analysis indicates that the developed stratum corneum and marrow is also the key structural characteristics to antagonize the saline stress.[Conclusion] Our results provide a valuable approach to study the salt-tolerance mechanism of plant using structural botanical techniques,i.e.,crystal may become the identification characteristics of salt tolerant plant.
魏健盖玉红蔡立格陆静梅王光野
关键词:CRYSTAL
基于外部效益的吉林省耕地保护经济补偿标准的实证分析被引量:8
2013年
中国耕地面积有持续减少的趋势,耕地保护多功能效应外溢是主要原因之一。以耕地的外部性理论为依据,运用收益还原法估算了2011年吉林省耕地保护的生态服务价值、社会保障价值和国家粮食安全价值,并计算了吉林省耕地保护外部性价值和补偿额度。在此基础上与其他省份和省市进行了对比分析,针对吉林省省情,提出了相关建议并归纳总结了相关结论。
于洋杨光张今华
关键词:外部性耕地保护经济补偿
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