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国家重点基础研究发展计划(2009CB118400)

作品数:50 被引量:385H指数:11
相关作者:韩天富吴存祥邱丽娟常汝镇关荣霞更多>>
相关机构:中国农业科学院作物科学研究所东北农业大学河北农业大学更多>>
发文基金:国家重点基础研究发展计划国家自然科学基金国家高技术研究发展计划更多>>
相关领域:农业科学生物学经济管理理学更多>>

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50 条 记 录,以下是 1-10
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Cloning and Characterization of Putative Hd6 Ortholog Associated with Zea mays L. Photoperiod Sensitivity
2011年
The photoperiod response of lowering is critical to the survival and successful reproduction in crops. The Hd6 gene, encoding a protein similar to the Arabidopsis α-subunit of CASEIN KINASE2α (CK2α) has been reported to affect flowering time through the photoperiodic pathway in rice. In this study, the maize ortholog, ZmHd6, has been cloned and its role in the photoperiodic pathway was investigated. ZmHd6 encodes 332 amino acids with six distinct domains: two CK2 active domains, a nuclear localization signal sequence, a N terminal domain, an ATP binding site, and an active site of serine/threonine protein kinase. ZmHd6 was constitutively expressed in the leaves and shoot apical meristem (SAM) with the highest expression in SAMs of plants with seven leaves under long and short day conditions. Delayed flowering time under long day (LD) conditions were observed in ZmHd6 overexpression transgenic Arabidopsis plants compared to the wild type. In transgenic Arabidopsis plants, ZmHd6 was expressed in a circadian oscillatory pattern, TOC1 peak expression was delayed, new GI, CO, and FT expressions reached to peak after 3 h while entering illumination period and oscillatory expression patterns of CO and FT were created during the dark phase. These results suggested that ZmHd6 might negatively regulate flowering time under LD conditions by delaying TOC1 peak expression and disrupting CO and FT expression in transgenic Arabidopsis plants.
KU Li-xia LI Si-yuan CHEN Xiao WU Lian-cheng WANG Xin-tao CHEN Yan-hui
关键词:EXPRESSION
Genome-Wide Expression Profile of Maize Root Response to Phosphorus Deficiency Revealed by Deep Sequencing被引量:1
2014年
Phosphorus (P) is one of the three primary macronutrients that are required in large amounts for plant growth and development. To better understand molecular mechanism of maize and identify relevant genes in response to phosphorus deficiency, we used Solexa/Illumina's digital gene expression (DGE) technology to investigate six genome-wide expression profiles of seedling roots of the low-P tolerant maize inbred line 178. DGE studies were conducted at 6, 24 and 72 h under both phosphorus deficient and sufficient conditions. Approximately 3.93 million raw reads for each sample were sequenced and 6 816 genes exhibited significant levels of differential expressions in at least one of three time points in response to P starvation. The number of genes with increased expression increased over time from 6 to 24 h, whereas genes with decreased expression were more abundant at 72 h, suggesting a gradual response process for P deficiency at different stages. Gene annotations illustrated that most of differentially expressed genes (DEGs) are involved in different cellular and molecular processes such as environmental adaptation and carbohydrate metabolism. The expression of some known genes identified in other plants, such as those involved in root architecture, P metabolism and transport were found to be altered at least two folds, indicating that the mechanisms of molecular and morphological adaptation to P starvation are conserved in plants. This study provides insight into the general molecular mechanisms underlying plant adaptation to low-P stress and thus may facilitate molecular breeding for improving P utilization in maize.
SU Shun-zhongWU LingLIU DanLU Yan-liLIN Hai-jianZHANG Shu-zhiSHEN Ya-ouLIU Hai-lanZHANG Zhi-mingRONG Ting-zhaoZHANG XiaoTIAN Yue-huiNIE ZhiGAO Shi-bin
关键词:MAIZEROOT
人工诱发创制大豆皱叶、类病突变体及其发育特性研究
物理或化学诱变育种技术作为创造作物新变异的重要途径,可使诱变群体基因突变频率大幅度提高,较易获得所需要的变异,特别在保持原有亲本特性、改良单一性状上尤为优越,已在主要农作物遗传育种研发中得到广泛应用。本文根据黄化、花色、...
聂智星代金英李丛丛赵团结
关键词:大豆诱变叶解剖结构
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Mapping of QTL Associated with Drought Tolerance in a Semi-Automobile Rain Shelter in Maize (Zea mays L.)被引量:5
2011年
Drought is a major constraint in maize production worldwide. We studied quantitative trait loci (QTL) underlying drought tolerance for maize plants grown in two different environments. Traits investigated included ASI, plant height, grain yield, ear height, and ear setting. A genetic linkage map was constructed with 120 simple sequence repeat (SSR) markers based on an F2 population derived from a cross between D5 (resistant parent) and 7924 (susceptible parent). Correlation and heritability were calculated. QTLs of these traits were identified by composite interval mapping combined with a linkage map covering 1 790.3 cM. The markers were arranged in ten linkage groups. QTL mapping was made of the mean trait performance of the 180 F2:3 population. The results showed five, five, six, four, and five QTLs for ASI, plant height, grain yield, ear height, and ear setting under full irrigation condition, respectively, and four, seven, six, four, and four QTLs for ASI, plant height, grain yield, ear height, and ear setting under severe late stress conditions, respectively. Especially the four QTLs detected for five traits in 2008 and 2009. The universal QTLs information generated in this study will aid in undertaking an integrated breeding strategy for further genetic studies in drought tolerance improvement in maize.
ZHU Jing-jing WANG Xiao-peng SUN Cui-xia ZHU Xiu-miao LI Meng ZHANG Guo-dong TIAN Yan-chen WANG Ze-li
关键词:MAIZE
国家大豆区域试验品种(系)SSR位点纯合度分析被引量:15
2012年
利用30对SSR引物,检测2005—2009年参加国家区域试验的1068份大豆品种(系)的位点纯合度。每年区试品种(系)的平均纯合度为94.9%~97.6%,纯合度高于85%和90%的品种(系)所占比例分别为95.0%和91.4%,纯合度低于85%的品种(系)有42份(占3.93%),主要为北方春大豆和黄淮夏大豆。位点纯合度低于85%的参试品种(系)的产量分析结果表明,只有11份品种(系)比对照增产5%以上,20份比对照减产0.04%~13.08%;与纯合度为100%的品种(系)比较发现,位点纯合度较低的品种(系)在区试中产量也较低。建议国家区试品种(系)纯合度标准不低于90%,以保证审定品种的特征特性,为大豆新品种的持续推广利用提供科技支撑。
关荣霞方宏亮何艳琴常汝镇邱丽娟
关键词:大豆SSR位点
利用生物信息学方法进行基于表达序列标签的玉米单核苷酸多态性标记的开发被引量:7
2010年
针对简单重复序列(SSR)标记密度不足、单核苷酸多态性(SNP)标记开发一般只基于2种基因型的序列差异,用以检测其他基因型的材料时多态性不高,不能满足玉米基因精细定位需要的现状,本研究从公共数据库下载来自不同遗传背景的玉米表达序列标签(EST),结合运用各种生物信息学软件,开发基于EST序列的高多态性SNP标记。通过对2018530条EST序列的比对分析,拼接发掘出遍布全基因组的80363个SNP位点。在SNP位点两侧保守序列上设计PCR引物,开发出12388个SNP标记(www.sicau.edu.cn/web/yms/snp/snp.html),包含34721个SNP位点。其中,6008个标记只含单一SNP位点,12762个位点的多态性信息含量(PIC)大于0.4,具有高度多态性。
吴玲付凤玲李晚忱刘海岚
关键词:表达序列标签玉米
玉米穗行数QTL及其互作分析被引量:5
2011年
利用与穗行数有关的5个导入系及轮回亲本综3进行GriffingⅣ双列杂交发展分离群体,结合SSR标记和田间表型鉴定,分析玉米穗行数QTL及其相互作用。在导入系×综3所发展的5个F2群体中,仅在一个群体中检测到1个穗行数QTL,所解释的表型变异为10.68%。在导入系间杂交所发展的F2群体中检测到9个QTLs,分别位于第13、、8染色体上,所解释的表型变异在4.53%~6.52%之间。另外,检测到2对QTL间互作,10对QTL与未检测到QTL的导入片段间的互作,单个F2群体中各类互作所解释的表型变异显著大于QTL所解释的表型变异。这些结果表明,基因互作在玉米穗行数形成中起着重要的作用。
李成璞白苇翟立红陶勇生张祖新
关键词:穗行数数量性状位点上位性
Growth Dynamics and Optimal Harvesting Stage of Two Forage Maize Varieties
2011年
SAUMZ 1 and SAUMZ 2 are two forage maize varieties reproduced by distant hybridization.In a randomized block experiment,their fresh forage yield and dry matter yield were investigated on the basis of growth period,leaf stage,and effective accumulated temperature.The nutritional components were measured in a near infrared diffuse reflectance spectroscopy.Referring to the concept of forage grading index,a formula was constructed to calculate the total digestible energy.The investigated characters and indexes were adapted to suitable models of growth dynamics.The results showed that these two varieties have strong heterosis in fresh forage yield,dry matter yield,and total digestible energy.Their growth dynamics were adapted to Logistic models described by general equation y=k/(1+ae-bx).The forage grading indexes of these two varieties were decreased linearly along with the increasing of their leaf stage.The stopping point of fast growing period of total digestible energy estimated on the basis of leaf stage was suggested to be used as optimal harvesting stage of forage maize.The optimal harvesting stage was estimated to be tasseling stage for SAUMZ 1 and 19.50-leaf stage for SAUMZ 2.
FU Feng-ling GUO Chang-jun TANG Qi-ling LIU Jian LI Wan-chen
以光周期处理与分期播种试验综合鉴定大豆品种的光温反应被引量:41
2009年
设置短日照(12h)和长日照(16h)两种光周期处理,并以春播模拟低温、夏播模拟高温条件,形成长日+低温、长日+高温、短日+低温、短日+高温4种光温组合。2007年对近年育成的10个北方春大豆[Glycinemax(L.)Merr.]品种(系)和18个黄淮海夏大豆品种(系)进行了光温反应特性鉴定。2008年对50份材料进行了光周期反应鉴定。结果表明,不论在低温(春播)还是高温(夏播)条件下,短日照均加快大豆的发育进程,导致开花提前;不论在长日照还是短日照条件下,高温均减少出苗至初花的日数。光周期和温度对大豆的发育存在明显的互作,随着温度的升高,短日照促进大豆发育的效应有所加强;随着日照的缩短,高温加快发育的作用也有所增大。供试大豆品种生态类型在光周期反应敏感度(PRS)、温度反应敏感度(TRS)及光温综合反应敏感度(PTCRS)等方面均存在显著差异。北方春大豆品种的上述3个指标均小于黄淮海夏大豆品种,但前者在不同光照条件下的温度反应敏感度差值和在不同温度条件下的光周期反应敏感度差值均较后者高,说明北方春大豆品种光温互作效应较强。
费志宏吴存祥孙洪波侯文胜张宝石韩天富
关键词:生态类型光周期
大豆苗期耐盐性的遗传及QTL定位分析被引量:8
2011年
利用亲本为科丰1号和南农1138-2的重组自交系群体NJRIKY进行大豆苗期耐盐性的遗传及QTL定位分析。以每个家系的平均存活时间为耐盐指标,采用主基因+多基因混合遗传模型进行RIL遗传分析,结果表明,NJRIKY群体的耐盐性遗传符合F-3模型,即由3对主基因控制,没有多基因修饰,主基因遗传率是64.4%。利用Cartographer V.2.5进行QTL定位,结果显示,共检测到3个耐盐QTL,它们分别位于B1、G和K三个连锁群上,分别解释8.4%、17.9%和11.3%的表型变异。
陈华涛陈新喻德跃
关键词:大豆耐盐性QTL
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