ζ （zeta）-carotene desaturase （ZDS） is a key enzyme for carotenoid biosynthesis, demonstrating high association with the yellow pigment （YP） content in wheat grain. Cloning ZDS gene and developing functional markers are important for marker-assisted selection in wheat breeding. In the present study, the full-length DNA sequence of a ZDS gene on wheat chromosome 2A, designated TaZds-A1, was cloned, with 14 exons and 13 introns, and it has an open reading frame （ORF） of 1 707 bp, encoding 568 amino acid residues. A co-dominant functional marker, YP2A-1, was designed based on the polymorphisms of two alleles at the locus, TaZds-Ala and TaZds-Alb, yielding 183- and 179-bp fragments in TaZds-Ala and TaZds-Alb genotypes, respectively. A new QTL for YP content was detected on chromosome 2A, co-segregating with the functional marker YP2A-1 and TaZds-A1; it explained 11.3% of the phenotypic variance for YP content in a doubled haploid （DH） population from Zhongyou 9507/CA9632. Among 217 Chinese wheat cultivars and advanced lines, the average grain YP content of 126 cultivars with TaZds-Alb allele was 7.8% higher than that of 91 cultivars with TaZds- Ala allele.
In this work,9 novel LMW-GS genes(6 LMW-m and 3 LMW-i type) from 4 diploid and 1 tetraploid Aegilops species w...
Shunli Wang~(1,2),Di Chen ~(1,2),Xixi Shen ~(1,2),Zitong Yu~1,Ting Zhang~1,Shanshan Jiang~1,Yueming Yan~(1,3) 1 Key Laboratory of Genetics and Biotechnology,College of Life Science,Capital Normal University,100048 Beijing,China
