Purpose： Microgravity is known to cause endothelium dysfunction in astronauts returning from spaceflight. We aimed to reveal the regulatory mechanism in alterations of human endothelial cells after simulated microgravity （SMG）. Methods： We utilized the rotary cell culture system （RCCS-1） to explore the subsequent effects of SMG on human umbilical vein endothelial cells （HUVECs）. Results： SMG-treated HUVECs appeared obvious growth inhibition after return to normal gravity, which might be attributed to a set of responses including alteration of cytoskeleton, decreased cell adhesion capacity and increased apoptosis. Expression levels of mTOR and its downstream Apaf-1 were increased during subsequent culturing after SMG. miR-22 was up-regulated and its target genes SRF and LAMC1 were down-regulated at mRNA levels. LAMC1 siRNAs reduced cell adhesion rate and inhibited stress fiber formation while SRF siRNAs caused apoptosis. Conclusion： SMG has the subsequent biological effects on HUVECs, resulting in growth inhibition through mTOR signaling and miR-22-mediated mechanism.
空间辐射是长期载人航天飞行任务中影响航天员健康的重要风险因素。为了探求载人探月过程中对空间辐射的合理防护方式,文章借助空间辐射场模型对"嫦娥三号"飞行任务在不同质量厚度材料屏蔽下的舱内空间辐射环境进行了仿真计算,并确定了航天员各器官接受的空间辐射剂量、剂量当量以及有效剂量等辐射防护量以进行辐射风险评估。结果表明,随着屏蔽厚度的增加,航天员的各组织或器官的吸收剂量和剂量当量以及有效剂量均明显降低;采用质量屏蔽的方法对低于100 Me V的质子具有很好的防护效果,但对高能质子或重离子的防护效果不明显。计算和分析显示,载人探月过程中,只要采取适当的防护措施,航天员的空间辐射风险是可控的。
