为探索地域及部位对烤烟成熟鲜烟叶化学成分的影响,采用红花大金元品种在昆明烟区海拔1920和1650 m 2个试点对3个部位成熟鲜烟叶的10种化学成分进行同步研究。结果表明,①总糖、还原糖含量在叶片中自下而上的增减趋势与海拔关系密切,高海拔点呈递减趋势,低海拔点呈递增趋势,而其他8种成分的变化趋势2个点基本一致,主要受叶片生长部位控制。②成熟鲜烟叶蛋白质、总多酚和总氮含量在叶片中自下而上呈递增趋势,淀粉含量呈"抛物线"变化,下部叶和中部叶两地含量差异较小,仅上部叶含量差异较大。③成熟鲜烟叶石油醚提取物、烟碱、挥发碱和挥发酸含量均随部位的升高而递增,两地间的含量差异是上部叶最大,中部叶次之,下部叶最小。
通过不同质量分数的磷酸三钠溶液震荡浸泡烟草种子30 m in,对种子的出苗和大田烟株TMV的发病情况进行调查统计.结果表明:1)30%和40%的磷酸三钠溶液对增强种子的活力有明显效果,播种后14 d出苗率分别达到63.5%和69.2%,分别比清水对照提高了13.9%和19.6%;2)30%和40%的磷酸三钠溶液处理种子30 m in,可使大田烟株对TMV有显著的抑制效果,与其它处理的差异显著水平达到0.01;3)大田期烟株TMV的病情变化趋势,几乎不受磷酸三钠溶液处理的影响,而主要与降雨等气候因素关系密切.
[ Objective] The paper was explore the control effects of combination of plant induced resistant agents against tobacco mosaic vires （TMV）. [ Method ] The control effects of 6 different combinations of plant induced resistant agents against TMV of flue-cured tobacco cultivar HangDa were studied under the environ- ment of simulated disease nursery. [ Result] The combination of 2 induced agents polypeptide-agent and 3-acetonyl-3-hydroxyoxindole （AHO） had good control effect against TMV, which could obviously delay the incidence time of TMV in infected tobacco plants. With water and Duxiao as control, their average control effects against TMV of tobacco plants during field period reached 69.64% and 43.25% after transplanting for 70 d. They also showed significant superiority accord- ing to Duncan＇s test （p = 0.05 ） in the aspects of plant height and leaf number, and the growth and development condition of leaves was good. Tobacco seedlings carrying TMV virus had no direct correlation with whether the symptoms performed, the seedlings carrying virus would perform symptom only when the incidence condition was suitable. The peak period for the incidence of TMV in seedlings carrying virus was during 19 d after transplanting. Spraying effective agents during nursery stage and field period, as well as promoting quick growth at the initial stage of tobacco seedlings after transplanting were the key measures to control its inci- dence. [ Conclusion] The study provided theoretical basis for preparing the control measures against TMV.
