The dynamic distribution of three different glycoconjugates in oocytes and follicle cells during the oogenesis of Atractomorpha sinensis were detected using biotin-labeled Peanut Agglutinin （PNA）, Soy Bean Agglutinin （SBA） and Ulex Europaeus Agglutinin I （UEA-I） lectins. The results showed that during oogenesis there was no distribution of the UEA-I receptor. The receptors of PNA and SBA were found to be dependent on developmental stage and present different distribution patterns accordingly. The binding sites of the two lectins indicated the presence of different sugars （PNA for Galβ1,3GalNAc and SBA for GalNAc） and showed considerable variation during oogenesis. PNA and SBA receptors first appeared at the oocyte growth phase, the PNA receptors then disappeared gradually and the SBA receptors exhibited the greatest expression. At the early phase of yolk formation, PNA and SBA receptors were located just at the brim of ooplasm, which was the region of vitellin formation. However at the later phase of yolk formation, neither of the two receptors was detected. In the mature egg, PNA and SBA receptors were distributed again on the vitellin membrane and the eggshell. The two receptors were also widely distributed in the follicular cells, showing similar distribution variation to the oocytes. The results indicate that the change and modification of the two receptors may be greatly related to the growth of oocytes, the preparation for yolk formation, the differentiation of follicular cells and the maturation of oocytes. The glycoconjugates on the vitellin membrane probably play important roles in sperm and egg recognition. The two lectins bound moderately or strongly to the eggshell, which indicates that the eggshell of A. sinensis contains the GalNAc and GalβGalNAc glycoproteins.
利用凝集素细胞化学方法对短额负蝗Atractomorpha sinensis Bolivar配子发生过程中蓖麻凝集素(Ricinus communis agglutininⅠ,RCA-Ⅰ)受体分布进行了定位研究,旨在认识昆虫配子发生过程中β-半乳糖复合物的分布规律及意义。结果表明:在短额负蝗精子发生过程中,RCA-Ⅰ在各期生精细胞膜上均有明显的阳性反应,变形期及成熟期精子头部为中强度阳性反应。卵子发生中,各阶段卵母细胞均为阳性反应,在卵母细胞生长期阳性反应达峰值,随着卵黄颗粒的逐渐形成,阳性反应逐渐减弱,成熟卵子中卵黄膜上重新出现中强度阳性反应。结果提示短额负蝗配子发生过程中β-半乳糖复合物的高度表达和修饰变化与其配子发生密切相关。
