Biomass is a key parameter in fermentation process, directly influencing the performance of the fermentation system as well as the quality and yield of the targeted product. Hybrid soft-sensor modeling is a good method for on-line estimation of biomass. Structure of hybrid soft-sensor model is a key to improve the estimating accuracy. In this paper, a forward heuristic breadth-first reasoning approach based on rule match is proposed for constructing structure of hybrid model. First, strategy of forward heuristic reasoning about facts is introduced, which can reason complex hybrid model structure in the event of few known facts. Second, rule match degree is defined to obtain higher esti- mating accuracy. The experiment results of Nosiheptide fermentation process show that the hybrid modeling process can estimate biomass with higher accuracy by adding transcendental knowledge and partial mechanism to the process.
Biomass is a key factor in fermentation process, directly influencing the performance of the fermentation system as well as the quality and yield of the targeted product. Therefore, the on-line estimation of biomass is indispensable. The soft-sensor based on support vector machine （SVM） for an on-line biomass estimation was analyzed in detail, and the improved SVM called the weighted least squares support vector machine was presented to follow the dynamic feature of fermentation process. The model based on the modified SVM was developed and demonstrated using simulation experiments.
Biomass is a key factor in fermentation process, directly influencing the performance of the fermenta- tion system as well as the quality and yield of the targeted product. Therefore, the on-line estimation of biomass is indispensable. The soft-sensor based on support vector machine (SVM) for an on-line biomass estimation was ana- lyzed in detail, and the improved SVM called the weighted least squares support vector machine was presented to follow the dynamic feature of fermentation process. The model based on the modified SVM was developed and demonstrated using simulation experiments.
State estimation is the precondition and foundation of a bioprocess monitoring and optimal control. However,there are many difficulties in dealing with a non-linear system,such as the instability of process, un-modeled dynamics,parameter sensitivity,etc.This paper discusses the principles and characteristics of three different approaches,extended Kalman filters,strong tracking filters and unscented transformation based Kalman filters.By introducing the unscented transformation method and a sub-optimal fading factor to correct the prediction error covariance,an improved Kalman filter,unscented transformation based robust Kalman filter,is proposed. The performance of the algorithm is compared with the strong tracking filter and unscented transformation based Kalman filter and illustrated in a typical case study for glutathione fermentation process.The results show that the proposed algorithm presents better accuracy and stability on the state estimation in numerical calculations.
In this study, Saccharomyces cerevisiae (baker's yeast) was produced in a fed-batch bioreactor at the optimal dissolved oxygen concentration (DOC) and growth medium temperature. However, it is very difficult to control the DOC using conventional controllers because of the poorly understood and constantly changing dynamics of the bioprocess. A generalized predictive controller (GPC) based on a nonlinear autoregressive integrated moving average exogenous (NARIMAX) model is presented to stabilize the DOC by manipulation of air flow rate. The NARIMAX model is built by an improved recursive least-squares support vector machine, which is trained by an in-place computation scheme and avoids the computation of the inverse of a large matrix and memory reallocation. The proposed nonlinear GPC algorithm requires little preliminary knowledge of the fermentation process, and directly obtains the nonlinear model in matrix form by using iterative multiple modeling instead of linearization at each sampling period. By application of an on-line bioreactor control, experimental results demonstrate the robustness, effectiveness and advantages of the new controller.
An expert system for biomass soft-sensor hybrid modeling in fermentation process was decribed in this paper.A production rules representation based on database was presented.The definitions of production rules for biomass soft-sensor hybrid modeling knowledge were proposed.A knowledge base with layered structure was introduced.A breadth-first reasoning approach based on match degree(BFMD) was developed.The definition and calculation method of match degree were illustrated.Compared with the depth-first reasoning approach based on exhaustive method(DFEM),the BFMD needs fewer introduced variables.This expert system could reduce the reasoning steps effectively,and advance reasoning efficiency.Tests shows that reasoning efficiency of the expert system using BFMD in the knowledge base with layered structure is improved 12.9% averagely,compared with using DFEM in the knowledge base with ranking structure.
根据发酵过程生物量检测特点,基于虚拟仪器技术提出了一种全新的生物量在线检测系统集成方法,该系统采用PXI(PCI Extensions for Instrumentation)总线硬件平台,结合软测量算法,有效地实现了生物量在线估计。给出了该系统的集成原理和实现方法,及系统的硬件和软件设计。实验研究表明,集成的发酵过程生物量软测量虚拟仪器系统,能够充分利用软测量技术和虚拟仪器技术的各自优势,系统硬件配置灵活,实用性强,并能得到较高的生物量在线测量精度。
