We label-free and real-time detected three interaction processes of antigen-antibodies,Human Immunoglobulin G(IgG),Rabbit IgG,and Mouse IgG as the targets,and Goat Anti-human IgG,Goat Anti-rabbit IgG,and Goat Anti-mouse IgG as the probe,by the Oblique-incidence Reflectivity Difference(OIRD) method.The interaction dynamic curves of the OIRD signal,corresponding to the interaction processes of antigen-antibodies,are generated.The reaction times from beginning to equilibrium state are about 1800,900,and 1200 s for Human IgG,Rabbit IgG,and Mouse IgG,respectively.The experimental results demonstrate that the OIRD method not only can distinguish biomolecular interactions,but also can be used in real-time detection of interactions and dynamic processes of biomolecules.
HE LiPingSUN YueDAI JunWANG JingYiLU HuiBinWANG ShuFangJIN KuiJuanZHOU YueLiangYANG GuoZhen
The entire interaction process between anti-mouse immunoglobulin-G (IgG) and mouse IgG with a concentration range from 1 to 0.25 mg/mL has been label-free detected by the oblique incidence reflectivity difference (OIRD) method.We achieved the two-dimensional scanning images of OIRD Re{△ p △s } intensities before and after interactions as well as the curves of reaction dynamic processes.The experimental results suggest that OIRD method not only can label-free distinguish the different concentrations of proteins and detect biological microarrays,but also can real-time monitor the kinetic processes of biomolecular interactions.
SUN YueHE LiPingDAI JunWANG JingYiLÜ HuiBinWANG ShuFangJIN KuiJuanZHOU YueLiangYANG GuoZhen
