激光扫描共聚焦显微镜（laser scanning confocal microscope,LSCM）是20世纪80年代发展的高科技产品,主要是采用激光作为光源,在荧光显微镜的基础上添加了激光扫描装置和计算机图像处理系统等,通过对观察样品进行光学断层扫描及三维结构重建等,可得到细胞或组织内部细微结构的荧光图像,同时激光扫描共聚焦显微镜还是活细胞动态观察、多重免疫荧光标记和Ca2＋荧光观察（Chen et al.,2009）的有力工具,目前已在花粉（Fang et al.,2008）和花粉管发育（Chen et al.,2008;Wu et al.,2008）、蛋白质功能（Zheng et al.,2009）及信号传导（Liu et al.。
[Objective]The aim was to research the relationship and genetic diversity of Indocalamus.[Method]Using 13 samples of Indocalamus and 3 samples of Sasa as materials,the intergenic regions of trnL-trnF gene in chloroplast were amplified by PCR,and sequence analysis and phylogenetic trees construction were carried out.[Result]Using the universal primer,the intergenic regions of trnL-trnF were amplified,the lengths of the segments varied from 1 008 bp to 1 103 bp,of which 940 bp was compared.The dendrogram of trnL-trnF sequences showed that Indocalamus and Sasa were clustered together and they were homologous by 99%.All the samples were divided into five groups,the first group included 12 samples such as Indosalamus pedalis,I.pumilus,I.victorialis,I.longiauritus,I.tessellatus,Sasa sinica,Sasa pygmaea,I.barbatus,I.guangdongensis,I.herklotsii,I.Hirtivaginatus and S.fortunei.I.decorus,I.lacunosus,I.Latifolius and I.Migoi were respectively divided into four groups.[Conclusion]The high homology of all samples showed the low evolution speed and little information sites which suggested that the phylogeny of Indocalamus could not be well resolved by the intergenic region of trnL-trnF.
