公共文化服务平台

共 2 条记录，以下是 1-5

全选清除导出

排序方式：

Molecular Cloning and Functional Analysis of GLO-1 and GLO-4 in Rice （Oryza sativa）: ＜正＞Glycolate oxidase （GLO） is known to be a key enzyme in photorespiration. Bioinformatic analysis shows that ...; Rongshu Deng, Xinxiang Peng* Lab of Molecular Plant Physiology, College of Life Sciences, South China Agricultural University, Guangzhou 510642, China; 关键词：RICE OVER-EXPRESSION; 文献传递

Inducible Antisense Suppression of Glycolate Oxidase Reveals its Strong Regulation over Photosynthesis in Rice: ＜正＞Photorespiration is one of the most intensively studied topics in plant biology. While a number of mutants ...; Huawei Xu1,3, Jianjun Zhang1,2, Xinxiang Peng1,2* 1Laboratory of Molecular Plant Physiology, College of Life Sciences, South China Agricultural University, Guangzhou 510642, China 2Key Laboratory of Plant Functional Genomics and Biotechnology, Education Department of Guangdong Province, South China Agricultural University; 关键词：PHOTORESPIRATION; 文献传递

Glyoxylate Rather than Ascorbate Is an Efficient Precursor for Oxalate Biosynthesis in Rice: ＜正＞Oxalate is widely distributed in plant kingdom. While excess oxalate in food plants is known to be detrimen...; Le Yu*, Jingzhe Jiang*, Chan Zhang*, Nenghui Ye, Ee Liu, Jianjun Zhang, Xinxiang Peng College of Life Sciences, South China Agricultural University, Guangzhou 510642, China; 关键词：ASCORBATE GLYCOLATE GLYOXYLATE OXALATE RICE; 文献传递

光呼吸突变体研究进展被引量：7: 2010年; 光呼吸(photorespiration)是绿色植物在光下吸收氧气并释放CO2的过程。C3植物光呼吸可消耗25%光合产物,故合理改良光呼吸可望提高植物的光合效率。筛选与利用光呼吸突变体是研究光呼吸代谢及其功能的最为有效的途径。该文对光呼吸代谢途径、光呼吸突变体的筛选以及研究进展进行综述,以期为深入探讨植物光呼吸的生物学功能及进行植物分子改良提供帮助。; 胥华伟姜敬哲彭新湘; 关键词：代谢途径突变体光呼吸光合作用

水稻L-半乳糖内酯脱氢酶基因的克隆和原核表达及抗体的制备被引量：4: 2010年; 应用RT-PCR技术从水稻叶片中克隆了水稻L-半乳糖内酯脱氢酶(GLDH)的全长基因.序列分析表明,水稻GLDH基因全长1752bp,亚克隆其部分成熟蛋白编码基因(789bp),利用基因重组技术构建了E.coli/pET30a-GLDHe,经酶切鉴定,DNA测序,证实重组质粒构建正确;将重组质粒转化大肠杆菌Rosseta,IPTG诱导表达,SDS-PAGE分析证实表达出相对分子质量约为36000的蛋白.用原核表达蛋白作为抗原免疫家兔制备抗体,用Westernblot检测抗体的特异性及效价,结果表明,抗体血清效价为1∶1000,在加IPTG诱导后的菌液中可以检测到相应的蛋白条带((相对分子质量为36000)),在水稻叶片样品中能检测到1条相对分子质量为36000的蛋白条带.; 俞乐刘拥海彭新湘; 关键词：水稻原核表达抗体

全选清除导出

共1页<1>

国家自然科学基金(30870184)