Two novel polyhydroxylated steroidal sapogenins, wattigenin B ((25R)-spirost-1beta, 2beta, 3beta, 4beta, 5beta, 6beta, 7alpha-heptol, 1) and wattigenin C ((25S)-spirost-1beta, 2beta, 3beta, 4beta, 5beta, 7alpha-hexalrydroxyl-6-one, 2), together with two known sapogenins, kitigenin (3) and convallagenin B (4), were isolated froth the fresh rhizomes of Tupistra wattii Hook. f. The structures of the compounds were determined on the basis of spectroscopic analysis. The four sapogenins were evaluated for the cytotoxicities on the cancer cell line K562 and A2780a in vitro. Compounds 1 - 4 were obtained from the plant for the first time.
Five compounds were isolated from mycelia of A.awamori ,and identified as ergosta 5,7,22 trien 3 β ol(Ⅰ),hexadecanoic acid(Ⅱ),ergosta 7,22 diene 3 β,5α,6β triol(Ⅲ), 5α,8α epidioxyergosta 6,22 diene 3 β ol(Ⅳ)and ergosta 7,22 diene 3,6 dione(Ⅴ)on the basis of spectral data and physical and chemical identification respectively.Among them,compound V was obtained from this genus for the first time.
