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国家自然科学基金(30300447)

作品数:3 被引量:87H指数:3
相关作者:赵淑娟胡之璧刘涤章国瑛更多>>
相关机构:上海中医药大学更多>>
发文基金:国家自然科学基金更多>>
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丹参水溶性酚酸类化合物药理及生物合成途径研究进展被引量:54
2004年
丹参具有活血化瘀、安神宁息之功效 ,是中医临床治疗冠心病、脑血栓、肝炎及肝硬化等的常用药。最新的药理研究表明 ,丹参的水溶性酚酸类化合物对肝癌、糖尿型肾病、抑郁和艾滋病 (AIDS)等具有潜在的治疗作用 ,是有待进一步开发的药用资源。对此类化合物生物合成途径的研究 ,可以为更好地利用这一资源奠定基础。
赵淑娟章国瑛刘涤胡之璧
关键词:丹参生物合成途径
Two Divergent Members of 4-Coumarate: Coenzyme A Ligase from Salvia miltiorrhiza Bunge: cDNA Cloning and Functional Study被引量:14
2006年
4-Coumarate : coenzyme A Ilgase (4CL) Is one of the key enzymes In phenylpropanoid metabolism leading to series of phenollcs, Including water-soluble phenolic acids, which are important compounds determining the medicinal quality of Danshen (Salvia miltiorrhiza Bunge), a traditional Chinese medicinal herb. To Investigate the function of 4CL in the biosynthesis of water-soluble phenolic acid in Danshen, we have cloned two cDNAs (Sm4CL1 and Sm4CL2) encoding divergent 4CL members by applying nested reverse transcrlptlon-polymerase chain reaction (RT-PCR) with degenerate primers followed by 5′/3′rapid amplification of cDNA ends (RACE) (Note, these sequence data have been submitted to the GenBank database under accession numbers AY237163 and AY237164). Either of the coding regions was inserted into a pRSET vector and a kinetic assay was performed with purified recombinant proteins. The substrate utilization profile of Sm4CL1 was distinct from that of Sm4CL2. The Km values of Sm4CL1 and Sm4CL2 to 4-coumarlc acid were (72.20±4.10) and (6.50±1.45) μmol/L, respectively. These results, In conjunction with Northern blotting and other information, imply that Sm4CL2 may play an Important role in the biosynthesis of watersoluble phenolic compounds, whereas Sm4CL1 may play a minor role in the pathway. Southern blotting analysis suggested that both Sm4CL1 and Sm4CL2 genes are present as a single copy and are located at different sites In the genome.
Shu-Juan ZhaoZhi-Bi HuDI LiuFrederick C. C. Leungt
植物4-香豆酸:辅酶A连接酶被引量:22
2006年
就植物中4-香豆酸:辅酶A连接酶的基因表达调控和底物结合功能域及其与木质素的关系以及其基因家族的进化的研究进展做了介绍。
赵淑娟刘涤胡之璧
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