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国家民委发酵酿造工程生物技术重点实验室资助项目(2008SY011)

作品数:1 被引量:2H指数:1
相关作者:段金华乌日娜刘雅琴更多>>
相关机构:北方民族大学更多>>
发文基金:国家民委发酵酿造工程生物技术重点实验室资助项目更多>>
相关领域:轻工技术与工程化学工程更多>>

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Optimization of Fermentation Conditions of Acid Resistant α-amylase Producing Strain被引量:2
2010年
[Objective] The aim was to optimize the fermentation conditions of acid resistant α-amylase producing strain. [Method] Based on the selection of an acid resistant α-amylase producing strain,the fermentation conditions including C,N contents and initial pH of culture medium,seed age,inoculum size,rotation speed of shake flask and fermentation temperature were optimized. [Result] The optimum fermentation conditions for acid resistant α-amylase producing strain were:seed age 14 h,inoculum size 8%,initial pH 5.5,fermentation temperature 35 ℃,rotation speed 150 r/min,the volume of inoculum broth 25 ml,C content 1.0% and N content 0.5%. [Conclusion] Under the optimum fermentation conditions,α-amylase activity reached 31.4 U/ml,which was 65.3 % higher than that before optimization.
刘雅琴乌日娜段金华
关键词:ALPHA-AMYLASEFERMENTATION
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