Graphene oxide （GO）/Ag nanoparticle （NP） hybrids are obtained by in situ reduction of Ag NPs on GO sheets. In this letter, the influence of the conformation of GO sheets on the surface-enhanced Raman scattering （SERS） effect of GO/Ag NPs is investigated by covalently grafting folic acid （FA） molecules onto graphite sheets. SERS measurements are conducted in aqueous solutions with different pH values. Data show that the SERS signals of FA are pH dependent, consistent with the morphological changes of GO sheets.
Spectral bleedthrough （SBT） ratio is dependent on the level of fluorescence intensity in confocal imaging. Precision Frster resonance energy transfer （FRET） algorithm corrects SBT ratio according to fluorescence intensity and avoids over-or under-estimation of SBT ratio. In this letter, we propose a new method to accurately measure the FRET efficiency of FRET plasmid in single living cells by combining the calculation of SBT in precision FRET algorithm with E-FRET formulae. We also use this method to measure the FRET efficiency of FRET-Bid, and find that in healthy A549 cells it is about 15%, which is verified by FRET acceptor photobleaching method.
