The miRNA expression profile was initially established to investigate its corresponding function in human uveal melanoma. The miRNA expression profile in human uveal melanoma was analyzed by a micro chip technique.The hsa-miRNA expression between four uveal melanomas and four normal uveal tissues was compared.Based on the bioinformatic approach,chip data was analyzed to select out differentially expressed candidate hsa-miRNAs.Real-time quantitative PCR(RT-PCR) was used to confirm the candidate hsa-miRNAs expression in all samples.The results of miRNA microarray chips that matched with RT-PCR were considered as the miRNA expression which was significantly different between normal tissue and uveal melanomas.In four uveal melanomas,expressions of miRNA-20a,miRNA-106a,miRNA-17,miRNA-21,and miRNA-34a were significantly up-regulated,while miRNA-145 and miRNA-204 expression were significantly down-regulated.We used miRNA microarray analysis as a fast,efficient technology to study biological information.The differentially expressed miRNAs may be involved in uveal melanoma pathogenesis,and may help promote the diagnosis and treatment for uveal melanoma.
