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国家自然科学基金(31001139)

作品数:2 被引量:7H指数:1
相关作者:刘菁华史西志李德祥孙爱丽陈炯更多>>
相关机构:宁波大学更多>>
发文基金:国家自然科学基金国家教育部博士点基金更多>>
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Marine bacterium strain screening and pyrethroid insecticide-degrading efficiency analysis
2014年
A pyrethroid insecticide-degrading bacterium, strain HS-24, was isolated from an offshore seawater environment. The strain, which can degrade cypermethrin (CYP) and deltamethrin (DEL), was identified as Methylophaga sp. The optimal culture and degradation conditions for CYP and DEL by strain HS-24 is pH 7 at 28℃. Under optimum culture conditions, strain HS-24 exhibited a broad degradation concentration range of 100, 200, 400, 600, and 800 mg/L for CYP and DEL. The metabolic intermediates were analyzed by NMR, which provided strong evidence that CYP and DEL removal occurred mainly because of a biological process. The toxicity of the degradation products of strain HS-24 was studied simultaneously by measuring the light output of the luminescence bacterium. This demonstrated that the biodegradation ability of strain HS-24 significantly decreased the toxicity of CYP- and DEL-contaminated aquaculture seawater. Finally, the findings of this paper indicate that strain HS-24 is thus revealed as a biological agent for the remediation of marine aquatic environments.
孙爱丽刘菁华史西志李德祥陈炯唐道军
分子印迹固相萃取-气相色谱法测定水产品中拟除虫菊酯类农药残留被引量:7
2013年
以氯氰菊酯(CYP)为模板分子,甲基丙烯酸(MAA)为功能单体,二甲基丙烯酸乙二醇酯(EGDMA)为交联剂,采用本体聚合方法制备得到对拟除虫菊酯类农药具有特异选择性的分子印迹聚合物(MIPs),以获得的MIPs作为吸附材料,建立了对5种拟除虫菊酯类农药具有高效富集、纯化能力的分子印迹固相萃取技术(MISPE)。结果表明,样品经MISPE净化处理,GC—ECD检测,水产品中5种拟除虫菊酯类农药的平均回收率为85.3~100.7%,相对标准偏差(RSD)为4.1~6.8%,最低检测限范围为16.1~26.5ng/kg,定量限范围为53.6~88.2ns/kg,可应用于水产样品中拟除虫菊酯类农药的定量检测。
刘菁华孙爱丽李德祥史西志陈炯
关键词:分子印迹聚合物菊酯类农药固相萃取
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