Objective： To identify serum biomarkers that may predict the short or long term outcomes of anti-Helicobacter gylori （H. pylori） treatment, a follow-up study was performed based on an intervention trial in Linqu County, China. Methods： A total of 529 subjects were selected randomly from 1,803 participants to evaluate total anti-H, pylori immunoglobulin G （IgG） and 10 specific antibody levels before and after treatment at 1-, 2- and 7.3-year. The outcomes of anti-H, pylori treatment were also parallelly assessed by 13C-urea breath test at 45-d after treatment and 7.3-year at the end of follow-up. Results： We found the medians of anti-H, pylori IgG titers were consistently below cut-off value through 7.3 years in eradicated group, however, the medians declined in recurrence group to 1.2 at 1-year after treatment and slightly increased to 2.0 at 7.3-year. While the medians were significantly higher （〉3.0 at 2- and 7.3-year） among subjects who failed the eradication or received placebo. For specific antibody responses, baseline seropositivities of FliD and HpaA were reversely associated with eradication failure [for FIiD, odds ratio （OR）=0.44, 95% confidence interval （95% CI）： 0.27-0.73; for HpaA, OR=0.32, 95% Ch 0.I7-0.60]. The subjects with multiple positive specific antibodies at baseline were more likely to be successfully eradicated in a linear fashion （Ptrend=0.006）. Conclusions： Our study suggested that total anti-H, pylori IgG level may serve as a potential monitor of long- term impact on anti-H, pylori treatment, and priority for H. pylori treatment may be endowed to the subjects with multiple seropositive antibodies at baseline, especially for FliD and HapA.
目的利用变性高效液相色谱（denaturing high performance liquid chromatography，DHPLC）技术，建立定量分析环氧合酶-2（cyclooxygenase-2，COX-2）基因启动子区甲基化水平的方法，并应用于胃黏膜组织甲基化状态的检测。方法利用亚硫酸氢钠-DHPLC技术，在55℃部分变性条件下，乙腈洗脱浓度梯度为4．0／min时，对COX-2基因启动子重要区域甲基化水平进行检测，并对10例胃镜活检石蜡组织标本进行测定。结果以人早幼粒细胞白血病细胞系（HL-60）和人胃癌细胞系（MGC803）分别作为完全甲基化和非甲基化对照，建立COX-2基因启动子甲基化定量分析的标准体系。甲基化含量百分率与相应色谱峰比值具有良好的线性关系，回归方程为Y=1．0608×甲基化色谱峰高（M）／甲基化和非甲基化色谱峰高之和（M＋U），R^2=0．9894。在此标准体系下测定10例胃黏膜组织标本中COX-2基因启动子甲基化含量百分率，发现甲基化阳性的2例异型增生病变中COX-2启动子甲基化含量百分率均高于甲基化阳性浅表性胃炎和慢性萎缩性胃炎（24．5％、18．4％对7．6％、9．6％）。结论本研究建立了COX-2启动子甲基化含量百分率定量分析体系，并首次将其应用于小样本胃黏膜组织标本的检测，为大规模筛查人群胃黏膜COX-2甲基化水平奠定了基础。
