Objective：The study was designed to investigate the molecular mechanism of quercitrin on osteogenic differentiation and adipogenic differentiation of r BMSCs.Methods：r BMSCs were harvested from SD rats,and determination of alkaline phosphatase（ALP）activity,quantification of mineralization by Alizarin Red S staining,and the m RNA expression of osteogenic differentiation markers（Runx2,BMP-2,and OSX）by RT-PCR after r BMSCs stimulated by osteogenic induction with（0.1–10）μg/m L of quercitrin,quantification of Lipid droplet by Oil Red O staining and the m RNA expression of adipogenic differentiation marker（,and a P2）by RT-PCR after r BMSCs stimulated by adipogenic induction with（0.1-10）μg/m L of quercitrin.Results：Quercitrin can up-regulate the m RNA expression of osteogenic differentiation markers（Runx2,BMP-2,and OSX）and increase ALP activity and mineralization after osteogenic induction,on the other hand quercitrin can suppress the m RNA expression of adipogenic differentiation markers（,and a P2）and decrease lipid droplet after adipogenic induction.Conclusion：This study suggested that quercitrin not only stimulated osteogenic differentiation but also inhibited adipogenic differentiation of r BMSCs,which was associated with the up-regulation of Runx2,BMP-2,and OSX m RNA expression and the down-regulation of,and a P2 m RNA expression.
Zi-yi GuanLan-ying ChenXue-liang LiYa-ru CuiRong-hua Liu
