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国家自然科学基金(30671322)

作品数:2 被引量:11H指数:2
相关作者:潘玉欣马骏韩改英王省芬马峙英更多>>
相关机构:河北农业大学更多>>
发文基金:河北省自然科学基金国家自然科学基金国家重点基础研究发展计划更多>>
相关领域:农业科学更多>>

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cDNA-AFLP profiling for the fiber development stage of secondary cell wall synthesis and transcriptome mapping in cotton被引量:5
2007年
Cotton fiber strength is mainly determined during the secondary cell wall deposition stage when cel- lulose is synthesized. We obtained cDNA of 20―25 d post anthesis (DPA) fiber from 109 F2 progeny and developed a cotton fiber transcriptome profiling via cDNA-AFLP technology using 37 different primer combinations. The F2 population originated from an interspecific cross between Gossypium hirsutum and Gossypium barbadense. One hundred and thirty-eight absence/presence polymorphic transcript- derived fragments (TDFs), with sizes ranging from 100 bp to 722 bp, were screened. Of these, 75 (53.62%) were polymorphic between the parents of the F2 population. Sequencing the 75 transcripts revealed that 37 of them had been reported to be cotton fiber ESTs. Nine of 75 transcript sequences were homologous to 7 cloned cotton fiber genes, encoding cysteine proteinase, vacuolar H+-pyro- phosphatase, vacuolar H+-ATPase, catalytic subunit, arabinogalactan protein, putative receptor protein kinase PERK1, GIA/RGA-like gibberellin response modulator and cellulose synthase. Some other transcripts may represent new gene fragments in cotton fiber development. Surprisingly, 46 of the 75 transcripts were mapped to a single linkage group. The transcriptome groups and the sequenced TDFs could serve as important resources in the functional genomic research of cotton fiber development.
PAN YuXin MA Jun ZHANG GuiYin HAN GaiYing WANG XingFent MA ZhiYingt
关键词:CDNA-AFLP基因转录
棉纤维次生壁加厚期cDNA-AFLP表达谱剖析和转录组图谱构建被引量:6
2007年
棉纤维强度主要由棉纤维次生壁加厚期决定.以陆地棉品种中棉所8号和海岛棉品种Pima90-53建立的包含109个单株的种间杂交F2群体为材料,用cDNA-AFLP对棉纤维发育次生壁加厚期(20~25DPA)基因表达谱进行剖析,并构建了次生壁加厚期转录组图谱.37对AFLP引物组合共检测到138个多态性表达片段(transcript-derived fragments,TDFs),大小在100~722bp之间,其中75个TDFs(53.62%)在亲本和F2单株中均具有多态性.在75个TDFs中,37个和已发表的棉纤维EST同源性较高:9个和已克隆的棉纤维基因,如半胱氨酸蛋白激酶、液泡H^+-焦磷酸激酶、液泡H^+-ATP催化亚基、半乳糖蛋白、受体蛋白激酶、GIA/RGA-赤霉素应答元件和纤维素合成酶等的编码基因同源性高;其他TDFs可能代表棉纤维发育过程中的新基因片段.在75个TDFs中,有46个集中分布在一个连锁群上。
潘玉欣马骏张桂寅韩改英王省芬马峙英
关键词:棉花CDNA-AFLP纤维发育
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