Nucleases play an important role in molecular biology, for example, in DNA sequencing. Synthetic polyamide conjugates can be considered as a novel tool for the selective inhibition of gene expressions and also as potential drugs in anticancer or antiviral chemotherapy. In this article, the synthesis of a novel minor-groove targeting artificial nuclease, an oligopyrrol-containing compound, has been reported. It was found that this novel compound can bind DNA in AT-rich minor groove with high affinity and site specificity. DNA binding behavior was determined by using UV-Vis and CD. It is indicated that compound 6 can enhance the Tm, of DNA from 80.4 ℃ to 84. 4 ℃ and that it possesses a high binding constant value（Kb =3.05 × 10^4 L/mol）.
＜正＞Molecular recognition of ATP by proteins,a subject of great importance for understanding enzymatic mechanis...
Jihong Liu~(a,b),Shuxia Cao~(a,*),Daxiong Hanc,Zhiping Zeng~c,Jiansha Lu~a,Xincheng Liao~a,Yufen Zhaoa,~(c,d*) a Key Laboratory of Chemical Biology and Organic Chemistry,Department of Chemistry,Zhengzhou University,Zhengzhou,450052,P R.China b Research Center of Agricultural Quality Standards and Testing Techniques,Henan Academy for Agricultural Sciences,Zhengzhou,450002,P R.China c The Key Laboratory for Chemical Biology of Fujian Province,Department of Chemistry and College of Chemistry and Chemical Engineering,Xiamen University,Xiamen 361005,P R China d The Key Laboratory of Bioorganic Phosphorus Chemisty & Chemical Biology,Ministry of Education, Department of chemistry,Tsinghua University,Beijing,100084,P R.China
Five multinuclear cyclotriphosphazene ligands were synthesized and tested for their cleavage activities to plasmid DNA. All of these new compounds were confirmed by MS, ^1H NMR, ^31P NMR, ^13C NMR and IR. Preliminary studies on the cleavage of pUC19 DNA in the presence of metal complexes were performed. The results revealed that these complexes could act as powerful catalysts under physiological conditions. The complexes 3b ＋ Cu can effectively cleave DNA to nicked form, giving hydrolysis rate constant of 0.08/h under physiological conditions. An acid-base catalyzed DNA phosphate-diester hydrolysis mechanism was also orooosed.
The DNA binding and cleavage activity of a synthetic polyamide containing dipeptide Ser-His has been investigated by spectroscopic techniques,such as electronic absorbance and circular dichroism(CD)spectropolarimetry,as well as gel electrophoresis.The results show that the molecule has a strong interaction with DNA and can improve DNA-cleavage ability about 100 fold as compared with single dipeptide Ser-His.
