The apatite(U-Th)/He thermochronometry has been used to study the tectono-thermal evolution of mountains and sedimentary basins for over ten years.The closure temperature of helium is important for the apatite(U-Th)/He thermochronometry and has been widely studied by thermal simulation experiments.In this paper,the apatite He closure temperature was studied by establishing the evolutionary pattern between apatite He ages and apatite burial depth based on examined apatite He ages of natural samples obtained from drillholes in the Tarim basin,China.The study showed that the apatite He closure temperature of natural samples in the Tarim basin is approximately 88±5℃,higher than the result(~75℃) obtained from the thermal simulation experiments.The high He closure temperature resulted from high effective uranium concentration,long-term radiation damage accumulation,and sufficient particle radii.This study is a reevaluation of the conventional apatite He closure temperature and has a great significance in studying the uplifting events in the late period of the basin-mountain tectonic evolution,of which the uplifting time and rates can be determined accurately.