Brain function depends on patterns of synaptic input and neurocircuits, which are accompanied by transient changes in free intracellular calcium concentration （Resendez et al., 2015）. A recent topic of interest has been the illumination of neuronal circuit activity by calcium imaging in vivo. As a new biological tool for recording behavioral signals in the animal brain, in vivo calcium imaging is performed to mon- itor neuronal activity based on intracellular calcium. In vivo calcium imaging is an advanced technique by which indi- vidual cells and/or even subcellular compartments can be observed in real time. Thus, it readily enables simultaneous recording of individual cells and subcellular compartments.
目的探讨帕金森病患者嗅觉障碍的发生率和特点及其可能的影响因素。方法采用12项气味识别能力测试(12 Item odor identification test from Sniffin’Sticks,SS-12)对106例帕金森病患者和110名正常志愿者进行嗅觉评估,比较两组的嗅觉功能,分析年龄、性别、文化程度、吸烟史、帕金森病病程、Hohn-Yahr分期、UPDRS-Ⅲ评分、左旋多巴用量与嗅觉的相关性。结果帕金森病组嗅觉得分(5.97±2.27)明显低于对照组(8.04±2.00),差异有统计学意义(P=0.000);帕金森病组对一些气味的识别(薄荷、香蕉、甘草、咖啡、菠萝、玫瑰、鱼)明显差于对照组(P<0.05);ROC曲线分析显示,7.5分是嗅觉障碍的最佳诊断界值,其敏感度为67.3%,特异度为73.6%,由此得出帕金森病组中嗅觉障碍的发生率为73.6%;相关性分析结果显示,帕金森病组性别(rs=-0.243)、文化程度(rs=0.208)及吸烟史(rs=-0.279,)与气味识别能力相关(P<0.05),而年龄、病程、Hohn-Yahr分期、UPDRS-Ⅲ评分及左旋多巴用量与气味识别能力不相关(P>0.05)。结论帕金森病患者嗅觉障碍发生率较高,帕金森病患者的嗅觉功能与疾病病程、Hohn-Yahr分期、UPDRS-Ⅲ评分及左旋多巴用量无关。
